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Literature summary for 3.1.6.14 extracted from

  • Xu, S.; Zhao, L.; Larsson, A.; Smeds, E.; Kusche-Gullberg, M.; Venge, P.
    Purification of a 75 kDa protein from the organelle matrix of human neutrophils and identification as N-acetylglucosamine-6-sulphatase (2005), Biochem. J., 387, 841-847.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.6.14

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
13
-
 N-acetyl-D-glucosamine 6-sulfate pH 7.0, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
primary granule 26% in fractions of secondary granules, 63% in fractions of primary granules Homo sapiens
-
-
secondary granule 26% in fractions of secondary granules, 63% in fractions of primary granules Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
granulocyte
-
 Homo sapiens
-
neutrophil
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.922
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glucose 6-sulfate + H2O about 55% of the activity with N-acetyl-D-glucosamine 6-sulfate Homo sapiens glucose + sulfate
-
 ?
additional information the enzyme shows O-desulphation activity towards heparan sulphate-derived saccharides. No activity with glucose 3-sulfate, galactose 4-sulfate, galactose 6-sulfate and N-acetylgalactosamine 4-sulfate Homo sapiens ?
-
 ?
N-acetyl-D-glucosamine 6-sulfate + H2O
-
 Homo sapiens N-acetyl-D-glucosamine + sulfate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 75000, SDS-PAGE under reducing and non-reducing conditions Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Homo sapiens