BRENDA - Enzyme Database show
show all sequences of 3.1.6.14

Prevotella enzymes involved in mucin oligosaccharide degradation and evidence for a small operon of genes expressed during growth on mucin

Wright, D.P.; Rosendale, D.I.; Robertson, A.M.; FEMS Microbiol. Lett. 190, 73-79 (2000)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Prevotella sp.
16020
-
periplasm
-
Prevotella sp.
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
119000
-
SDS-PAGE
Prevotella sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Prevotella sp.
-
-
-
Prevotella sp. RS2
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Prevotella sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.00044
-
cell extract after growth on galactose
Prevotella sp.
0.00086
-
cell extract after growth on mucin
Prevotella sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-acetyl-D-glucosamine 6-sulfate + H2O
in heparan
651356
Prevotella sp.
N-acetyl-D-glucosamine + sulfate
-
651356
Prevotella sp.
?
N-acetyl-D-glucosamine 6-sulfate + H2O
in heparan
651356
Prevotella sp. RS2
N-acetyl-D-glucosamine + sulfate
-
651356
Prevotella sp. RS2
?
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Prevotella sp.
16020
-
periplasm
-
Prevotella sp.
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
119000
-
SDS-PAGE
Prevotella sp.
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Prevotella sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.00044
-
cell extract after growth on galactose
Prevotella sp.
0.00086
-
cell extract after growth on mucin
Prevotella sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
N-acetyl-D-glucosamine 6-sulfate + H2O
in heparan
651356
Prevotella sp.
N-acetyl-D-glucosamine + sulfate
-
651356
Prevotella sp.
?
N-acetyl-D-glucosamine 6-sulfate + H2O
in heparan
651356
Prevotella sp. RS2
N-acetyl-D-glucosamine + sulfate
-
651356
Prevotella sp. RS2
?
Other publictions for EC 3.1.6.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729709
Zhou
Neutralizing the anticoagulant ...
Homo sapiens
FEBS J.
280
2523-2532
2013
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1
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704598
Myette
Heparin/heparan sulfate 6-O-su ...
Pedobacter heparinus
J. Biol. Chem.
284
35177-35188
2009
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-
1
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3
1
3
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1
1
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2
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4
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3
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4
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1
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1
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677855
Jansen
Sanfilippo syndrome type D: na ...
Homo sapiens
Arch. Neurol.
64
1629-1634
2007
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1
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1
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1
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663957
Xu
Purification of a 75 kDa prote ...
Homo sapiens
Biochem. J.
387
841-847
2005
-
-
-
-
-
-
-
1
2
-
1
-
-
2
-
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1
-
-
2
1
-
3
1
-
-
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-
1
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-
-
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-
-
-
-
-
-
-
1
2
-
1
-
-
-
-
1
-
2
1
-
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
652758
Beesley
Sanfilippo syndrome type D: id ...
Homo sapiens
J. Med. Genet.
40
192-194
2003
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
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1
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1
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1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651356
Wright
Prevotella enzymes involved in ...
Prevotella sp., Prevotella sp. RS2
FEMS Microbiol. Lett.
190
73-79
2000
-
-
-
-
-
-
-
-
2
-
1
-
-
3
-
-
1
-
-
-
2
-
2
-
-
-
-
-
-
-
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2
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1
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1
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2
-
2
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-
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-
-
-
-
-
-
-
-
-
-
135592
Medeiros
New pathway of heparan sulphat ...
Tagelus gibbus
Comp. Biochem. Physiol. B
119
539-547
1998
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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1
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-
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2
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1
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1
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2
-
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-
-
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-
-
-
-
-
-
-
-
-
135593
Sleat
alpha-Glucosidase and N-acetyl ...
Homo sapiens
Biochem. J.
324
33-39
1997
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
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1
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1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135594
Litjens
Expression, purification and c ...
Capra hircus
Biochem. J.
327
89-94
1997
-
1
1
-
1
-
-
2
-
-
2
1
-
1
-
-
1
-
-
-
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
2
-
-
2
1
-
-
-
1
-
-
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
135591
Shilatifard
Purification and characterizat ...
Bos taurus
Biochemistry
33
4273-4282
1994
-
-
-
-
-
1
2
1
-
-
3
1
-
3
-
-
1
-
-
1
-
2
2
-
1
-
2
-
1
1
1
-
-
-
-
-
-
-
-
-
-
1
-
2
-
1
-
-
3
1
-
-
-
1
-
1
-
2
2
-
1
-
2
-
1
1
1
-
-
-
-
-
-
-
135588
Thompson
N-Acetylglucosamine 6-sulphata ...
Capra hircus
J. Inherit. Metab. Dis.
15
760-768
1992
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
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-
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1
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135589
Robertson
A cDNA clone for human glucosa ...
Homo sapiens
Biochem. J.
288
539-544
1992
-
-
1
-
-
-
-
-
-
-
3
1
-
1
-
1
1
-
-
1
-
-
3
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
1
-
-
1
1
-
1
-
-
3
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135590
Freeman
Human glucosamine-6-sulphatase ...
Homo sapiens
Biochem. J.
282
605-614
1992
1
-
-
-
-
-
-
3
-
-
1
1
-
1
-
-
1
-
-
4
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
1
1
-
-
-
1
-
4
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
135586
Freeman
Sanfilippo D syndrome: estimat ...
Homo sapiens
Anal. Biochem.
176
244-248
1989
1
1
-
-
-
-
4
3
1
-
-
1
-
2
-
-
-
-
-
7
-
-
3
-
-
-
-
-
3
-
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
3
1
-
-
1
-
-
-
-
-
7
-
-
3
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
135579
Freeman
Human liver N-acetylglucosamin ...
Homo sapiens
Biochem. J.
246
347-354
1987
-
-
-
-
-
-
-
3
1
-
6
1
-
1
-
1
1
-
-
1
3
1
6
3
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
1
-
6
1
-
-
1
1
-
1
3
1
6
3
-
-
-
-
1
-
3
-
-
-
-
-
-
-
135580
Freeman
Human liver N-acetylglucosamin ...
Bos taurus, Homo sapiens, Rattus norvegicus
Biochem. J.
246
355-365
1987
1
-
-
-
-
1
5
4
-
-
1
2
-
3
-
-
-
-
-
5
-
-
8
-
-
-
-
-
4
-
-
-
-
-
-
1
-
-
-
-
-
1
-
5
-
4
-
-
1
2
-
-
-
-
-
5
-
-
8
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
135582
Elliott
Detection of the Sanfilippo D ...
Homo sapiens
Anal. Biochem.
138
205-209
1984
-
1
-
-
-
-
2
1
-
-
-
1
-
1
-
-
-
-
-
3
4
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
3
4
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
135585
Kresse
Enzymic diagnosis of the genet ...
Homo sapiens
Methods Enzymol.
83
559-572
1982
-
1
-
-
-
-
1
-
-
-
-
1
-
1
-
-
1
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
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-
-
1
-
-
-
1
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135584
Kresse
Liberation of N-acetylglucosam ...
Homo sapiens
J. Biol. Chem.
256
12926-12932
1981
-
1
-
-
-
-
-
-
-
-
-
2
-
1
-
-
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-
1
-
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3
-
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-
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1
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2
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1
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3
-
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-
-
-
135583
Di Ferrante
N-acetylglucosamine-6-sulfate ...
Homo sapiens
Science
210
448
1980
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
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2
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1
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1
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1
-
-
2
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
135587
Kresse
Sanfilippo disease type D: def ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
77
6822-6826
1980
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
3
-
-
-
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-
-
-
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1
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1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
135581
Basner
N-Acetylglucosamine-6-sulfate ...
Homo sapiens
J. Biol. Chem.
254
1151-1158
1979
-
-
-
-
-
-
7
2
-
-
1
1
-
1
-
1
1
-
-
7
-
-
8
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
-
1
1
-
-
1
1
-
7
-
-
8
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-