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Literature summary for 3.1.6.14 extracted from

  • Litjens, T.; Bielicki, J.; Anson, D.S.; Friderici, K.; Jones, M.Z.; Hopwood, J.J.
    Expression, purification and characterization of recombinant caprine N-acetylglucosamine-6-sulphatase (1997), Biochem. J., 327, 89-94.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.6.14

Application

Application Comment Organism
medicine endocytosis of the recombinant enzyme by human fibroblasts Capra hircus

Cloned(Commentary)

Cloned (Comment) Organism
expression in CHO cells Capra hircus

Protein Variants

Protein Variants Comment Organism
R102X nonsense mutation Capra hircus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0482
-
 O-(alpha-glucosamine 6-sulfate)-(1-3)-L-idonic acid
-
 Capra hircus
5.8
-
 4-Methylumbelliferyl sulfate
-
 Capra hircus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
10200
-
 of the recombinant protein Capra hircus
94000
-
 reduced, SDS-PAGE Capra hircus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-glucosamine 6-sulfate units + H2O Capra hircus
-
 N-acetyl-D-glucosamine units + sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Capra hircus
-
 Nubian goat, caprine
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant protein Capra hircus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl sulfate + H2O fluorogenic substrate Capra hircus 4-methylumbelliferone + sulfate
-
 ?
N-acetyl-D-glucosamine 6-sulfate units + H2O
-
 Capra hircus N-acetyl-D-glucosamine units + sulfate
-
 ?
O-(alpha-glucosamine 6-sulfate)-(1-3)-L-idonic acid + H2O
-
 Capra hircus O-(alpha-glucosamine)-(1-3)-L-idonic acid + sulfate
-
 ?
O-(alpha-N-acetyl glucosamine 6-sulfate)-(1-3)-L-idonic acid + H2O
-
 Capra hircus O-(alpha-N-acetyl glucosamine)-(1-3)-L-idonic acid + sulfate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
 Capra hircus
6
-
-
 Capra hircus