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Literature summary for 3.1.6.14 extracted from

  • Freeman, C.; Hopwood, J.J.
    Human glucosamine-6-sulphatase deficiency. Diagnostic enzymology towards heparin-derived trisaccharide substrates (1992), Biochem. J., 282, 605-614.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.6.14

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin stimulates only in urine at pH 3.9 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005 0.004 O-(alpha-glucosamine 6-sulfate)-(1-4)-L-O-(alpha-iduronic acid 2-sulfate)-(1-4)-D-O-2,5-anhydro mannitol 6-sulfate in urine depending on pH and presence of bovine serum albumin Homo sapiens
0.009 0.017 O-(alpha-glucosamine 6-sulfate)-(1-4)-L-O-(alpha-iduronic acid 2-sulfate)-(1-4)-D-O-2,5-anhydro mannitol 6-sulfate in fibroblasts Homo sapiens
0.028
-
 O-(alpha-glucosamine 6-sulfate)-(1-4)-L-O-(alpha-iduronic acid 2-sulfate)-(1-4)-D-O-2,5-anhydro mannitol 6-sulfate in leucocytes Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
 6 individual activites determined by chromatofocusing Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-glucosamine 6-sulfate units + H2O Homo sapiens in oligo-and monosaccharides derived from heparan sulfate and keratan sulfate N-acetyl-D-glucosamine units + sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
6 individual activites determined Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
leukocyte
-
 Homo sapiens
-
liver
-
 Homo sapiens
-
skin fibroblast in culture Homo sapiens
-
urine
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-glucosamine 6-sulfate units + H2O
-
 Homo sapiens N-acetyl-D-glucosamine units + sulfate
-
 ?
N-acetyl-D-glucosamine 6-sulfate units + H2O in oligo-and monosaccharides derived from heparan sulfate and keratan sulfate Homo sapiens N-acetyl-D-glucosamine units + sulfate
-
 ?
O-(alpha-glucosamine 6-sulfate)-(1-4)-L-O-(alpha-iduronic acid 2-sulfate)-(1-4)-D-O-2,5-anhydro mannitol 6-sulfate + H2O N-sulfated and N-acetylated derivates of Homo sapiens O-(alpha-glucosamine)-(1-4)-L-O-(alpha-iduronic acid 2-sulfate)-(1-4)-D-O-2,5-anhydro mannitol 6-sulfate + sulfate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.9
-
 O-(alpha-glucosamine 6-sulfate)-(1-4)-L-O-(alpha-iduronic acid 2-sulfate)-(1-4)-D-O-2,5-anhydro mannitol 6-sulfate in fibroblasts Homo sapiens
4.1
-
 O-(alpha-glucosamine 6-sulfate)-(1-4)-L-O-(alpha-iduronic acid 2-sulfate)-(1-4)-D-O-2,5-anhydro mannitol 6-sulfate in leucocytes Homo sapiens