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Literature summary for 3.1.6.14 extracted from

  • Freeman, C.; Clements, P.R.; Hopwood, J.J.
    Human liver N-acetylglucosamine-6-sulphate sulphatase. Purification and characterization (1987), Biochem. J., 246, 347-354.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.6.14

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0111
-
 O-(alpha-glucosamine 6-sulfate)-(1-3)-L-idonic acid form C Homo sapiens
0.0117
-
 O-(alpha-glucosamine 6-sulfate)-(1-3)-L-idonic acid form A Homo sapiens
0.0142
-
 O-(alpha-glucosamine 6-sulfate)-(1-3)-L-idonic acid form B Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 1 * 48000, 1 * 32000, form B, SDS-PAGE Homo sapiens
32000
-
 1 * 48000, 1 * 45000, 1 * 32000, form C, SDS-PAGE Homo sapiens
45000
-
 1 * 48000, 1 * 45000, 1 * 32000, form C, SDS-PAGE Homo sapiens
48000
-
 1 * 48000, 1 * 32000, form B, SDS-PAGE Homo sapiens
48000
-
 1 * 48000, 1 * 45000, 1 * 32000, form C, SDS-PAGE Homo sapiens
75000
-
 form B, gel filtration Homo sapiens
78000
-
 form A, gel filtration Homo sapiens
94000
-
 form C, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-glucosamine 6-sulfate units + H2O Homo sapiens in oligo-and monosaccharides derived from heparan sulfate and keratan sulfate N-acetyl-D-glucosamine units + sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 2 major forms: A, B , 2 minor form: C and D
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
form A and B, form C: partial Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0026
-
 form C Homo sapiens
0.23
-
 form A Homo sapiens
0.254
-
 form B Homo sapiens

Storage Stability

Storage Stability Organism
4°C, 2 months, pH 5.7, form A and B retain 95% of original activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-glucosamine 6-sulfate units + H2O specific exosulfatase for desulfation of the nonreducing terminal N-acetylglucosamine 6-sulfate residues of heparansulfate Homo sapiens N-acetyl-D-glucosamine units + sulfate
-
 ?
N-acetyl-D-glucosamine 6-sulfate units + H2O in oligo-and monosaccharides derived from heparan sulfate and keratan sulfate Homo sapiens N-acetyl-D-glucosamine units + sulfate
-
 ?
O-(alpha-2-sulfamino glucosamine 6-sulfate)-(1-4)-L-idonic acid + H2O
-
 Homo sapiens O-(alpha-2-sulfamino glucosamine)-(1-4)-L-idose + sulfate
-
 ?
O-(alpha-glucosamine 6-sulfate)-(1-3)-L-idonic acid + H2O
-
 Homo sapiens O-(alpha-glucosamine)-(1-3)-L-idonic acid + sulfate
-
 ?
O-(alpha-N-acetyl glucosamine 6-sulfate)-(1-3)-L-idonic acid + H2O
-
 Homo sapiens O-(alpha-N-acetyl glucosamine)-(1-3)-L-idonic acid + sulfate
-
 ?
O-(beta-N-acetyl glucosamine 6-sulfate)-(1-3)-D-galactitol + H2O activity 100-fold less Homo sapiens O-(alpha-N-acetyl glucosamine)-(1-3)-D-galactitol + sulfate
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 48000, 1 * 32000, form B, SDS-PAGE Homo sapiens
monomer 1 * 78000, form A, SDS-PAGE Homo sapiens
trimer 1 * 48000, 1 * 45000, 1 * 32000, form C, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 forms: A, B, C Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
 24 h, complete inactivation below pH 4.5 Homo sapiens
5 7.5
-
 Homo sapiens
9.5
-
 24 h, complete inactivation above pH 9.5 Homo sapiens