Cloned (Comment) | Organism |
---|---|
expression in HT-1080 human fibroblasts | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
structure with a covalently bound sulfate ion in the active site, to 2.3 A resolution | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P22304 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | deglycosylation with PNGase leads to a decrease in molecular mass from 76000 Da to 58000 Da | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.6 | - |
20°C, pH 5.0 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-alpha-L-iduronate 2-sulfate + H2O | - |
Homo sapiens | 4-methylumbelliferol + L-iduronate 2-sulfate | - |
? | |
additional information | mechanism follows a SN2 nucleophilic attack of the formylglycine FGH84 on the sulfur atom to generate the covalent formylglycine-S adduct, followed by elimination of bound sulfate and rehydration of the resultant formylglycine aldehyde. Residues H229, H138, D334 and K347 are likely to play active roles in proton transfer or activation of water during the reaction cycle | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 76000, SDS-PAGE | Homo sapiens |