Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.6.13 extracted from

  • Pimentel, N.; Rodriguez-Lopez, A.; Diaz, S.; Losada, J.C.; Diaz-Rincon, D.J.; Cardona, C.; Espejo-Mojica, A.J.; Ramirez, A.M.; Ruiz, F.; Landazuri, P.; Poutou-Pinales, R.A.; Cordoba-Ruiz, H.A.; Almeciga-Diaz, C.J.; Barrera-Avellaneda, L.A.
    Production and characterization of a human lysosomal recombinant iduronate-2-sulfatase produced in Pichia pastoris (2018), Biotechnol. Appl. Biochem., 65, 655-664 .
    View publication on PubMed
Search for more literature for 3.1.6.13

Application

Application Comment Organism
synthesis expression of protein in Pichia pastoris. The highest production of recombinant IDS is obtained at oxygen-limited conditions using a codon-optimized IDS cDNA.The purified enzyme shows a final activity of 12.45 nmol/mg/h. IDS shows high stability in human serum and is taken up by HEK-293 cells in a dose-dependent manner through mannose receptors Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P22304
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.21
-
 recombinant protein, pH not specified in the publication, temperature not specified in the publication Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-idopyranosiduronic acid 2-sulfate + H2O
-
 Homo sapiens 4-methylumbelliferyl-alpha-iduronate + sulfate
-
 ?

Subunits

Subunits Comment Organism
? x * 90000, SDS-PAGE, recombinant protein Homo sapiens

Synonyms

Synonyms Comment Organism
IDS
-
 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 highest stability Homo sapiens