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Literature summary for 3.1.6.13 extracted from

  • Cordoba-Ruiz, H.; Poutou-Pinales, R.; Echeverri-Pena, O.; Algecira-Enciso, N.; Landazuri, P.; Saenz, H.; Barrera-Avellaneda, L.
    Laboratory scale production of the human recombinant iduronate 2-sulfate sulfatase-Like from Pichia pastoris (2009), Afr. J. Biotechnol., 8, 1786-1792.
No PubMed abstract available

Application

Application Comment Organism
additional information improvement of the total activity of recombinant IDS-Like at 3 litre bioreactor in glycerol as carbon source. Clone IDS28 of Pichia pastoris expressing IDS-Like employed for low-scale production of the recombinant enzyme in a saline culture media without phosphate. Biological activity is about 1.73 to 7times higher in batch culture than the result obtained with the same clone in shake flask culture Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
Pichia pastoris strain GS115:His- transformed with the integrative plasmid pPIC9-hIDS-Like (Clone IDS28) Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-iduronide-2-sulfate + H2O
-
Homo sapiens 4-methylumbelliferyl-alpha-iduronide + sulfate
-
?

Synonyms

Synonyms Comment Organism
IDS-Like
-
Homo sapiens
iduronate 2-sulfate sulfatase-like
-
Homo sapiens

Expression

Organism Comment Expression
Homo sapiens IDS-Like expression is induced by methanol addition up

General Information

General Information Comment Organism
physiological function recombinant IDS-Like activity increases when passed from culture M6 (11.34 g/l of glycerol) to M7 (25.53 g/l of glycerol), from 14.7 to 29.5 nmol/h/mg of total protein, between the 33 and 47 h of culture. In M8 (40.13 g/l of glycerol), the recombinant IDS-Like activity is 7.3 nmol/h/mg of total protein at 47h of culture. In M9 (83.32 g/l of glycerol), no recombinant IDS-Like activity is detected. Recombinant IDS-Like activity is lower when extra-cellular proteolytic activity increases Homo sapiens