Application | Comment | Organism |
---|---|---|
additional information | improvement of the total activity of recombinant IDS-Like at 3 litre bioreactor in glycerol as carbon source. Clone IDS28 of Pichia pastoris expressing IDS-Like employed for low-scale production of the recombinant enzyme in a saline culture media without phosphate. Biological activity is about 1.73 to 7times higher in batch culture than the result obtained with the same clone in shake flask culture | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
Pichia pastoris strain GS115:His- transformed with the integrative plasmid pPIC9-hIDS-Like (Clone IDS28) | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-alpha-iduronide-2-sulfate + H2O | - |
Homo sapiens | 4-methylumbelliferyl-alpha-iduronide + sulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDS-Like | - |
Homo sapiens |
iduronate 2-sulfate sulfatase-like | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | IDS-Like expression is induced by methanol addition | up |
General Information | Comment | Organism |
---|---|---|
physiological function | recombinant IDS-Like activity increases when passed from culture M6 (11.34 g/l of glycerol) to M7 (25.53 g/l of glycerol), from 14.7 to 29.5 nmol/h/mg of total protein, between the 33 and 47 h of culture. In M8 (40.13 g/l of glycerol), the recombinant IDS-Like activity is 7.3 nmol/h/mg of total protein at 47h of culture. In M9 (83.32 g/l of glycerol), no recombinant IDS-Like activity is detected. Recombinant IDS-Like activity is lower when extra-cellular proteolytic activity increases | Homo sapiens |