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Literature summary for 3.1.6.12 extracted from

  • Gibson, G.J.; Saccone, G.T.P.; Brooks, D.A.; Clements, P.R.; Hopwood, J.J.
    Human N-acetylgalactosamine-4-sulphate sulphatase. Purification, monoclonal antibody production and native and subunit Mr values (1987), Biochem. J., 248, 755-764.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.6.12

Application

Application Comment Organism
medicine deficiency in Maroteaux-Lamy syndrome, mucopolysaccharidosis type IV Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 4-Methylumbelliferyl sulfate pH 3.5 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13000
-
 1 * 57000, composed of 2 peptides 1 * 43000, 1 * 13000 Homo sapiens
43000
-
 1 * 57000, composed of 2 peptides 1 * 43000, 1 * 13000 Homo sapiens
57000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-galactosamine 4-sulfate units + H2O Homo sapiens acts also on N-acetylglucosamine 4-sulfate N-acetyl-D-galactosamine units + sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.282
-
-
 Homo sapiens
93.3
-
 nitrocatechol sulfate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl sulfate + H2O
-
 Homo sapiens 4-methylumbelliferone + sulfate
-
 ?
N-acetygalactosamine 4-sulfate-(1-4)-beta-glucuronic acid-(1-3)-beta-N-acetylgalactosaminitol 4-sulfate + H2O oligosaccharides Homo sapiens N-acetygalactosamine-(1-4)-beta glucuronic acid-(1-3)-beta N-acetylgalactosaminitol 4-sulfate + sulfate
-
 ?
N-acetyl-D-galactosamine 4-sulfate units + H2O acts also on N-acetylglucosamine 4-sulfate Homo sapiens N-acetyl-D-galactosamine units + sulfate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 57000, composed of 2 peptides 1 * 43000, 1 * 13000 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8
-
 oligosaccharide substrate, formate buffer Homo sapiens
4.4
-
 oligosaccharide substrate, acetate buffer or dimethylglutarate buffer Homo sapiens
5.5
-
 4-methylumbelliferyl sulfate Homo sapiens