Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.61 extracted from

  • van Alebeek, G.; Kreuwels, M.; Keltjens, J.; Vogels, G.
    Methanobacterium thermoautotrophicum (strain DELTAH) contains a membrane-bound cyclic 2,3-diphosphoglycerate hydrolase (1994), Arch. Microbiol., 161, 514-520 .
No PubMed abstract available
Search for more literature for 3.1.4.61

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition at 1 mM, the activity can be restored by addition of 2 mM Co2+ Methanothermobacter thermautotrophicus
KCl 50% inhibition at 0.2 M, 90% at 1 M Methanothermobacter thermautotrophicus
O2 complete inhibition Methanothermobacter thermautotrophicus
phosphate 50% inhibition at 0.6-0.7 mM Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics determined with the single substrate model Methanothermobacter thermautotrophicus
59
-
 cyclic 2,3-bisphosphoglycerate pH 6.0, 60°C Methanothermobacter thermautotrophicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Methanothermobacter thermautotrophicus 16020
-
additional information a membrane-bound cDPG hydrolase is described in the organism. The properties of this isozyme differ very much from the soluble isozyme Methanothermobacter thermautotrophicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ dependent on Methanothermobacter thermautotrophicus
Mn2+ can replace Co2+ to 38% Methanothermobacter thermautotrophicus
additional information no activity with Mg2+, Ca2+, and Zn2+ Methanothermobacter thermautotrophicus
Ni2+ can replace Co2+ to 71% Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclic 2,3-bisphosphoglycerate + H2O Methanothermobacter thermautotrophicus
-
 2,3-diphosphoglycerate
-
 ?
cyclic 2,3-bisphosphoglycerate + H2O Methanothermobacter thermautotrophicus DSM 1053
-
 2,3-diphosphoglycerate
-
 ?
cyclic 2,3-bisphosphoglycerate + H2O Methanothermobacter thermautotrophicus DELTAH
-
 2,3-diphosphoglycerate
-
 ?

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-
Methanothermobacter thermautotrophicus DELTAH
-
-
-
Methanothermobacter thermautotrophicus DSM 1053
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native membrane-bound isozyme 27.2fold by ultracentrifugation (3 steps), solubilization through treatment with 25 mM CHAPS, followed by anion exchange and hydrophobic interaction chromatography, Ba2+ salt precipitation, washing with 60% acetone, and cation exchange chromatography Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30.5
-
 purified native enzyme, in presence of Co2+, pH 6.0, 60°C Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclic 2,3-bisphosphoglycerate + H2O
-
 Methanothermobacter thermautotrophicus 2,3-diphosphoglycerate
-
 ?
cyclic 2,3-bisphosphoglycerate + H2O
-
 Methanothermobacter thermautotrophicus DSM 1053 2,3-diphosphoglycerate
-
 ?
cyclic 2,3-bisphosphoglycerate + H2O
-
 Methanothermobacter thermautotrophicus DELTAH 2,3-diphosphoglycerate
-
 ?
additional information hydrolysis of cDPG occurs under nitrogen or hydrogen atmosphere Methanothermobacter thermautotrophicus ?
-
-
additional information hydrolysis of cDPG occurs under nitrogen or hydrogen atmosphere Methanothermobacter thermautotrophicus DSM 1053 ?
-
-
additional information hydrolysis of cDPG occurs under nitrogen or hydrogen atmosphere Methanothermobacter thermautotrophicus DELTAH ?
-
-

Subunits

Subunits Comment Organism
? x * 33000, SDS-PAGE Methanothermobacter thermautotrophicus

Synonyms

Synonyms Comment Organism
cDPG hydrolase
-
 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Methanothermobacter thermautotrophicus

General Information

General Information Comment Organism
metabolism cDPG biosynthesis and hydrolysis is related to the intermediary cell carbon (C3-)metabolism in Methanobacterium thermoautotrophicum. cDPG is synthesized from 2-phosphoglycerate (2-PG) in two subsequent ATP-dependent reactions by cDPG synthase (EC 6.5.1.9). In the intermediary cell carbon metabolism of methanogenic bacteria 2-PG is produced via phosphoenolpyruvate (PEP) from pyruvate, cDPG hydrolysis occurs by the hydrolysis of the diphosphate bonding, followed by the dephosphorylation of 2,3-DPG into phosphoglycerate (3-PG). 2-PG and 3-PG are interconverted by the action of phosphoglycerate mutase Methanothermobacter thermautotrophicus
physiological function the function of cDPG may be multiple, cDPG has been suggested to act in storage of energy, phosphorous, or cell carbon. Furthermore, cDPG may play a role in thermostabilization of proteins in extremely thermophilic archaebacteria Methanothermobacter thermautotrophicus