Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.60 extracted from

  • Rorvik, G.H.; Liskiewicz, K.A.; Kryuchkov, F.; Naemi, A.O.; Aasheim, H.C.; Petersen, F.C.; Kuentziger, T.M.; Simm, R.
    Cyclic di-adenosine monophosphate regulates metabolism and growth in the oral commensal Streptococcus mitis (2020), Microorganisms, 8, 1269.
    View publication on PubMed
Search for more literature for 3.1.4.60

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptococcus mitis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1485
-
 cyclic-di-3',5'-adenylate pH 8.5, 37°C Streptococcus mitis
0.877
-
 5'-O-phosphonoadenylyl-(3'->5')-adenosine pH 8.5, 37°C Streptococcus mitis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92000
-
 gel filtration Streptococcus mitis

Organism

Organism UniProt Comment Textmining
Streptococcus mitis
-
-
-
Streptococcus mitis CCUG 31611
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus mitis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O
-
 Streptococcus mitis 2 AMP
-
 ?
5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O
-
 Streptococcus mitis CCUG 31611 2 AMP
-
 ?
cyclic-di-3',5'-adenylate + H2O
-
 Streptococcus mitis 2 AMP
-
 ?
cyclic-di-3',5'-adenylate + H2O
-
 Streptococcus mitis CCUG 31611 2 AMP
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Streptococcus mitis

Synonyms

Synonyms Comment Organism
PDE2
-
 Streptococcus mitis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.078
-
 cyclic-di-3',5'-adenylate pH 8.5, 37°C Streptococcus mitis
3527
-
 5'-O-phosphonoadenylyl-(3'->5')-adenosine pH 8.5, 37°C Streptococcus mitis

General Information

General Information Comment Organism
malfunction the DELTApde2-mutant grows significantly slower in exponential phase compared to the wild-type and reaches a lower OD in stationary phase. Thes display a reduced rate of glucose metabolism Streptococcus mitis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.525
-
 cyclic-di-3',5'-adenylate pH 8.5, 37°C Streptococcus mitis
4021
-
 5'-O-phosphonoadenylyl-(3'->5')-adenosine pH 8.5, 37°C Streptococcus mitis