BRENDA - Enzyme Database
show all sequences of 3.1.4.60

Two DHH subfamily 1 proteins in Streptococcus pneumoniae possess cyclic di-AMP phosphodiesterase activity and affect bacterial growth and virulence

Bai, Y.; Yang, J.; Eisele, L.E.; Underwood, A.J.; Koestler, B.J.; Waters, C.M.; Metzger, D.W.; Bai, G.; J. Bacteriol. 195, 5123-5132 (2013)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0239
-
5'-O-phosphonoadenylyl-(3'->5')-adenosine
pH 7.5, 37°C
Streptococcus pneumoniae serotype 2
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
protein possesses two transmembrane helices
Streptococcus pneumoniae serotype 2
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
required
Streptococcus pneumoniae serotype 2
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptococcus pneumoniae serotype 2
A0A0H2ZNP2
-
-
Streptococcus pneumoniae serotype 2 D39
A0A0H2ZNP2
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O
-
750954
Streptococcus pneumoniae serotype 2
2 AMP
-
-
-
?
5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O
-
750954
Streptococcus pneumoniae serotype 2 D39
2 AMP
-
-
-
?
cyclic di-3',5'-adenylate + H2O
-
750954
Streptococcus pneumoniae serotype 2
5'-O-phosphonoadenylyl-(3'->5')-adenosine
-
-
-
?
additional information
Pde2 directly hydrolyzes c-di-AMP into AMP. Additionally, Pde2 degrades 5'-O-phosphonoadenylyl-(3'->5')-adenosine into AMP. Pde2 prefers substrate 5'-O-phosphonoadenylyl-(3'->5')-adenosine
750954
Streptococcus pneumoniae serotype 2
?
-
-
-
-
additional information
Pde2 directly hydrolyzes c-di-AMP into AMP. Additionally, Pde2 degrades 5'-O-phosphonoadenylyl-(3'->5')-adenosine into AMP. Pde2 prefers substrate 5'-O-phosphonoadenylyl-(3'->5')-adenosine
750954
Streptococcus pneumoniae serotype 2 D39
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 35000, calculated from sequence
Streptococcus pneumoniae serotype 2
More
protein possesses two transmembrane helices, a PAS domain, an atypical GGDEF domain, a DHH domain, and a DHHA1 domain
Streptococcus pneumoniae serotype 2
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Streptococcus pneumoniae serotype 2
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0239
-
5'-O-phosphonoadenylyl-(3'->5')-adenosine
pH 7.5, 37°C
Streptococcus pneumoniae serotype 2
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
protein possesses two transmembrane helices
Streptococcus pneumoniae serotype 2
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
required
Streptococcus pneumoniae serotype 2
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O
-
750954
Streptococcus pneumoniae serotype 2
2 AMP
-
-
-
?
5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O
-
750954
Streptococcus pneumoniae serotype 2 D39
2 AMP
-
-
-
?
cyclic di-3',5'-adenylate + H2O
-
750954
Streptococcus pneumoniae serotype 2
5'-O-phosphonoadenylyl-(3'->5')-adenosine
-
-
-
?
additional information
Pde2 directly hydrolyzes c-di-AMP into AMP. Additionally, Pde2 degrades 5'-O-phosphonoadenylyl-(3'->5')-adenosine into AMP. Pde2 prefers substrate 5'-O-phosphonoadenylyl-(3'->5')-adenosine
750954
Streptococcus pneumoniae serotype 2
?
-
-
-
-
additional information
Pde2 directly hydrolyzes c-di-AMP into AMP. Additionally, Pde2 degrades 5'-O-phosphonoadenylyl-(3'->5')-adenosine into AMP. Pde2 prefers substrate 5'-O-phosphonoadenylyl-(3'->5')-adenosine
750954
Streptococcus pneumoniae serotype 2 D39
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 35000, calculated from sequence
Streptococcus pneumoniae serotype 2
More
protein possesses two transmembrane helices, a PAS domain, an atypical GGDEF domain, a DHH domain, and a DHHA1 domain
Streptococcus pneumoniae serotype 2
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Streptococcus pneumoniae serotype 2
General Information
General Information
Commentary
Organism
physiological function
deletion of either isoform Pde1 or Pde2 results in a moderate increase of the c-di-AMP levels compared with the parental strain. Deletion of both genes results in an up to 4fold increase in c-di-AMP levels compared to that of the parental strain. Both Pde1 and Pde2 play a role in pneumococcal growth. Deletion of either isoform Pde1 or Pde2 reduces the growth rate slightly, and the double mutant synergizes the reduction
Streptococcus pneumoniae serotype 2
General Information (protein specific)
General Information
Commentary
Organism
physiological function
deletion of either isoform Pde1 or Pde2 results in a moderate increase of the c-di-AMP levels compared with the parental strain. Deletion of both genes results in an up to 4fold increase in c-di-AMP levels compared to that of the parental strain. Both Pde1 and Pde2 play a role in pneumococcal growth. Deletion of either isoform Pde1 or Pde2 reduces the growth rate slightly, and the double mutant synergizes the reduction
Streptococcus pneumoniae serotype 2
Other publictions for EC 3.1.4.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751077
Bowman
New insights into the cyclic ...
Staphylococcus aureus
J. Biol. Chem.
291
26970-26986
2016
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1
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3
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1
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3
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1
1
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-
750844
Tang
Functional analysis of a c-di ...
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis ATCC 700084
Int. J. Biol. Sci.
11
813-824
2015
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2
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4
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4
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2
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4
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2
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1
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2
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4
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4
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4
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2
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1
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1
1
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750731
Ye
DhhP, a cyclic di-AMP phospho ...
Borreliella burgdorferi, Borreliella burgdorferi DSM 4680
Infect. Immun.
82
1840-1849
2014
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2
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1
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1
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1
1
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750954
Bai
Two DHH subfamily 1 proteins ...
Streptococcus pneumoniae serotype 2, Streptococcus pneumoniae serotype 2 D39
J. Bacteriol.
195
5123-5132
2013
-
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1
1
1
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2
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5
2
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1
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1
1
1
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5
2
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1
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1
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