BRENDA - Enzyme Database
show all sequences of 3.1.4.60

Functional analysis of a c-di-AMP-specific phosphodiesterase MsPDE from Mycobacterium smegmatis

Tang, Q.; Luo, Y.; Zheng, C.; Yin, K.; Ali, M.; Li, X.; He, J.; Int. J. Biol. Sci. 11, 813-824 (2015)

Data extracted from this reference:

Engineering
Protein Variants
Commentary
Organism
D134a/H135A/H136A
mutation in DHH domain, complete loss of catalytic activity
Mycolicibacterium smegmatis
G313A/G314A/G315A/H316A
mutation in DHHA1 domain, mutant displays residual activity
Mycolicibacterium smegmatis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
Pde is strictly dependent on Co2+, Mn2+, Mg2+ or Fe2+, with maximum activity with Mn2+ as a cofactor
Mycolicibacterium smegmatis
Fe2+
Pde is strictly dependent on Co2+, Mn2+, Mg2+ or Fe2+, with maximum activity with Mn2+ as a cofactor
Mycolicibacterium smegmatis
Mg2+
Pde is strictly dependent on Co2+, Mn2+, Mg2+ or Fe2+, with maximum activity with Mn2+ as a cofactor
Mycolicibacterium smegmatis
Mn2+
Pde is strictly dependent on Co2+, Mn2+, Mg2+ or Fe2+, with maximum activity with Mn2+ as a cofactor
Mycolicibacterium smegmatis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O
Mycolicibacterium smegmatis
-
2 AMP
-
-
?
5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O
Mycolicibacterium smegmatis ATCC 700084
-
2 AMP
-
-
?
cyclic di-3',5'-adenylate + H2O
Mycolicibacterium smegmatis
-
5'-O-phosphonoadenylyl-(3'->5')-adenosine
-
-
?
cyclic di-3',5'-adenylate + H2O
Mycolicibacterium smegmatis ATCC 700084
-
5'-O-phosphonoadenylyl-(3'->5')-adenosine
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Mycolicibacterium smegmatis
A0QVM9
protein contains a DHH-DHHAI domain
-
Mycolicibacterium smegmatis ATCC 700084
A0QVM9
protein contains a DHH-DHHAI domain
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O
-
750844
Mycolicibacterium smegmatis
2 AMP
-
-
-
?
5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O
-
750844
Mycolicibacterium smegmatis ATCC 700084
2 AMP
-
-
-
?
cyclic di-3',5'-adenylate + H2O
-
750844
Mycolicibacterium smegmatis
5'-O-phosphonoadenylyl-(3'->5')-adenosine
-
-
-
?
cyclic di-3',5'-adenylate + H2O
-
750844
Mycolicibacterium smegmatis ATCC 700084
5'-O-phosphonoadenylyl-(3'->5')-adenosine
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
MSMEG_2630
-
Mycolicibacterium smegmatis
PDE
-
Mycolicibacterium smegmatis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
50
-
Mycolicibacterium smegmatis
37
-
assay at
Mycolicibacterium smegmatis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Mycolicibacterium smegmatis
Engineering (protein specific)
Protein Variants
Commentary
Organism
D134a/H135A/H136A
mutation in DHH domain, complete loss of catalytic activity
Mycolicibacterium smegmatis
G313A/G314A/G315A/H316A
mutation in DHHA1 domain, mutant displays residual activity
Mycolicibacterium smegmatis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
Pde is strictly dependent on Co2+, Mn2+, Mg2+ or Fe2+, with maximum activity with Mn2+ as a cofactor
Mycolicibacterium smegmatis
Fe2+
Pde is strictly dependent on Co2+, Mn2+, Mg2+ or Fe2+, with maximum activity with Mn2+ as a cofactor
Mycolicibacterium smegmatis
Mg2+
Pde is strictly dependent on Co2+, Mn2+, Mg2+ or Fe2+, with maximum activity with Mn2+ as a cofactor
Mycolicibacterium smegmatis
Mn2+
Pde is strictly dependent on Co2+, Mn2+, Mg2+ or Fe2+, with maximum activity with Mn2+ as a cofactor
Mycolicibacterium smegmatis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O
Mycolicibacterium smegmatis
-
2 AMP
-
-
?
5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O
Mycolicibacterium smegmatis ATCC 700084
-
2 AMP
-
-
?
cyclic di-3',5'-adenylate + H2O
Mycolicibacterium smegmatis
-
5'-O-phosphonoadenylyl-(3'->5')-adenosine
-
-
?
cyclic di-3',5'-adenylate + H2O
Mycolicibacterium smegmatis ATCC 700084
-
5'-O-phosphonoadenylyl-(3'->5')-adenosine
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O
-
750844
Mycolicibacterium smegmatis
2 AMP
-
-
-
?
5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O
-
750844
Mycolicibacterium smegmatis ATCC 700084
2 AMP
-
-
-
?
cyclic di-3',5'-adenylate + H2O
-
750844
Mycolicibacterium smegmatis
5'-O-phosphonoadenylyl-(3'->5')-adenosine
-
-
-
?
cyclic di-3',5'-adenylate + H2O
-
750844
Mycolicibacterium smegmatis ATCC 700084
5'-O-phosphonoadenylyl-(3'->5')-adenosine
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
50
-
Mycolicibacterium smegmatis
37
-
assay at
Mycolicibacterium smegmatis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Mycolicibacterium smegmatis
General Information
General Information
Commentary
Organism
physiological function
deficiency of Pde significantly enhances intracellular C12-C20 fatty acid accumulation. Superfluous c-di-AMP in Mycobacterium smegmatis may lead to abnormal colonial morphology
Mycolicibacterium smegmatis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
deficiency of Pde significantly enhances intracellular C12-C20 fatty acid accumulation. Superfluous c-di-AMP in Mycobacterium smegmatis may lead to abnormal colonial morphology
Mycolicibacterium smegmatis
Other publictions for EC 3.1.4.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751077
Bowman
New insights into the cyclic ...
Staphylococcus aureus
J. Biol. Chem.
291
26970-26986
2016
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
750844
Tang
Functional analysis of a c-di ...
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis ATCC 700084
Int. J. Biol. Sci.
11
813-824
2015
-
-
-
-
2
-
-
-
-
4
-
4
-
2
-
-
-
-
-
-
-
-
4
-
2
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
4
-
2
-
-
-
1
-
-
-
-
1
1
-
-
-
750731
Ye
DhhP, a cyclic di-AMP phospho ...
Borreliella burgdorferi, Borreliella burgdorferi DSM 4680
Infect. Immun.
82
1840-1849
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
2
-
-
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-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
750954
Bai
Two DHH subfamily 1 proteins ...
Streptococcus pneumoniae serotype 2, Streptococcus pneumoniae serotype 2 D39
J. Bacteriol.
195
5123-5132
2013
-
-
-
-
-
-
-
1
1
1
-
-
-
2
-
-
-
-
-
-
-
-
5
2
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
5
2
-
-
-
-
1
-
-
-
-
1
1
-
-
-