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Literature summary for 3.1.4.58 extracted from

  • Kanai, A.; Sato, A.; Fukuda, Y.; Okada, K.; Matsuda, T.; Sakamoto, T.; Muto, Y.; Yokoyama, S.; Kawai, G.; Tomita, M.
    Characterization of a heat-stable enzyme possessing GTP-dependent RNA ligase activity from a hyperthermophilic archaeon, Pyrococcus furiosus (2009), RNA, 15, 420-431 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.58

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(ribonucleotide)n-2',3'-cyclic phosphate + H2O Pyrococcus furiosus
-
 (ribonucleotide)n-2'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U4Q3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(ribonucleotide)n-2',3'-cyclic phosphate + H2O
-
 Pyrococcus furiosus (ribonucleotide)n-2'-phosphate
-
 ?
additional information protein possesses GTP-dependent RNA ligase activity and synthetic tRNA halves bearing 2',3'-cyclic phosphate and 5'-OH termini are substrates for the ligation reaction in vitro, reaction of EC 6.5.1. Protein shows strong CPDase activity, the RNA ligase activity is quite weak Pyrococcus furiosus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 21000, calculated, x * 22000, SDS-PAGE Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
CPDase
-
 Pyrococcus furiosus
PF0027
-
 Pyrococcus furiosus
RNA 2',3'-cyclic phosphodiesterase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pyrococcus furiosus