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Literature summary for 3.1.4.57 extracted from

  • Ghodge, S.V.; Cummings, J.A.; Williams, H.J.; Raushel, F.M.
    Discovery of a cyclic phosphodiesterase that catalyzes the sequential hydrolysis of both ester bonds to phosphorus (2013), J. Am. Chem. Soc., 135, 16360-16363 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.57

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 5-phospho-alpha-D-ribose 1,2-cyclic phosphate pH 8.0, 25°C Eggerthella lenta
0.023
-
 D-ribofuranose 2,5-bisphosphate pH 8.0, 25°C Eggerthella lenta

Organism

Organism UniProt Comment Textmining
Eggerthella lenta C8WJZ5
-
-
Eggerthella lenta DSM 2243 C8WJZ5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-phospho-alpha-D-ribose 1,2-cyclic phosphate + H2O
-
 Eggerthella lenta D-ribofuranose 2,5-bisphosphate
-
 ?
5-phospho-alpha-D-ribose 1,2-cyclic phosphate + H2O
-
 Eggerthella lenta DSM 2243 D-ribofuranose 2,5-bisphosphate
-
 ?
D-ribofuranose 2,5-bisphosphate + H2O
-
 Eggerthella lenta D-ribofuranose 5-phosphate + phosphate
-
 ?
D-ribofuranose 2,5-bisphosphate + H2O
-
 Eggerthella lenta DSM 2243 D-ribofuranose 5-phosphate + phosphate
-
 ?
additional information enzyme hydrolyzes the phosphate ester at carbon-1 of the ribose moiety of 5-phosphoribose-1,2-cyclic phosphate to form ribose-2,5-bisphosphate, and then this intermediate is hydrolyzed to ribose-5-phosphate and inorganic phosphate. Ribose-1,5-bisphosphate is neither an intermediate nor substrate for this enzyme Eggerthella lenta ?
-
 ?
additional information enzyme hydrolyzes the phosphate ester at carbon-1 of the ribose moiety of 5-phosphoribose-1,2-cyclic phosphate to form ribose-2,5-bisphosphate, and then this intermediate is hydrolyzed to ribose-5-phosphate and inorganic phosphate. Ribose-1,5-bisphosphate is neither an intermediate nor substrate for this enzyme Eggerthella lenta DSM 2243 ?
-
 ?

Synonyms

Synonyms Comment Organism
cPDH
-
 Eggerthella lenta
cyclic phosphate dihydrolase
-
 Eggerthella lenta
phnPP
-
 Eggerthella lenta

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 5-phospho-alpha-D-ribose 1,2-cyclic phosphate pH 8.0, 25°C Eggerthella lenta
7.4
-
 D-ribofuranose 2,5-bisphosphate pH 8.0, 25°C Eggerthella lenta

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
140
-
 5-phospho-alpha-D-ribose 1,2-cyclic phosphate pH 8.0, 25°C Eggerthella lenta
330
-
 D-ribofuranose 2,5-bisphosphate pH 8.0, 25°C Eggerthella lenta