Crystallization (Comment) | Organism |
---|---|
to 1.4 A resolution. The active site of PhnP contains probably two Mn2+ ions surrounded by an array of active site residues that are identical to those observed in the tRNase Z enzymes. A second, remote Zn2+ binding site is also observed, composed of a set of cysteine and histidine residues that are strictly conserved in the PhnP family. This second metal ion site appears to stabilize a structural motif | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D80A | inactive with substrate bis(p-nitrophenyl)phosphate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
2',3'-cAMP | pH 7.2, 25°C, presence of Mn2+ | Escherichia coli | |
0.14 | - |
2',3'-cCMP | pH 7.2, 25°C, presence of Mn2+ | Escherichia coli | |
0.31 | - |
2',3'-cGMP | pH 7.2, 25°C, presence of Mn2+ | Escherichia coli | |
0.68 | - |
bis(p-nitrophenyl)phosphate | pH 7.2, 25°C, presence of Zn2+ | Escherichia coli | |
2.3 | - |
bis(p-nitrophenyl)phosphate | pH 7.2, 25°C, presence of Ni2+ | Escherichia coli | |
2.9 | - |
bis(p-nitrophenyl)phosphate | pH 7.2, 25°C, presence of Mn2+ | Escherichia coli | |
15 | - |
thymidine 5'-monophosphate p-nitrophenyl ester | pH 7.2, 25°C, presence of Mn2+ | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | distinct preference for Mn2+ and Ni2+ for catalysis. 0.13 mol per mol of monomer | Escherichia coli | |
Ni2+ | preference for Mn2+ and Ni2+ for catalysis. 1.3 mol per mol of monomer | Escherichia coli | |
Zn2+ | poor activity with Zn2+ | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | P16692 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-cAMP + H2O | - |
Escherichia coli | 3'-AMP | - |
? | |
2',3'-cCMP + H2O | - |
Escherichia coli | 3'-CMP | - |
? | |
2',3'-cGMP + H2O | - |
Escherichia coli | 3'-GMP | - |
? | |
bis(p-nitrophenyl)phosphate + H2O | - |
Escherichia coli | ? | - |
? | |
additional information | highest activity is observed with 2',3'-cyclic nucleotides. This reaction is regiospecific, with only the 3'-nucleotide product being observed. No activity toward RNA, 3',5'-cyclic nucleotides, p-nitrophenyl phosphate | Escherichia coli | ? | - |
? | |
thymidine 5'-monophosphate p-nitrophenyl ester + H2O | - |
Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phnP | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
bis(p-nitrophenyl)phosphate | pH 7.2, 25°C, presence of Zn2+ | Escherichia coli | |
0.08 | - |
thymidine 5'-monophosphate p-nitrophenyl ester | pH 7.2, 25°C, presence of Mn2+ | Escherichia coli | |
0.64 | - |
2',3'-cCMP | pH 7.2, 25°C, presence of Mn2+ | Escherichia coli | |
1.19 | - |
bis(p-nitrophenyl)phosphate | pH 7.2, 25°C, presence of Mn2+ | Escherichia coli | |
1.23 | - |
2',3'-cGMP | pH 7.2, 25°C, presence of Mn2+ | Escherichia coli | |
1.71 | - |
2',3'-cAMP | pH 7.2, 25°C, presence of Mn2+ | Escherichia coli | |
4.1 | - |
bis(p-nitrophenyl)phosphate | pH 7.2, 25°C, presence of Ni2+ | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
thymidine 5'-monophosphate p-nitrophenyl ester | pH 7.2, 25°C, presence of Mn2+ | Escherichia coli | |
0.044 | - |
bis(p-nitrophenyl)phosphate | pH 7.2, 25°C, presence of Zn2+ | Escherichia coli | |
0.41 | - |
bis(p-nitrophenyl)phosphate | pH 7.2, 25°C, presence of Mn2+ | Escherichia coli | |
1.77 | - |
bis(p-nitrophenyl)phosphate | pH 7.2, 25°C, presence of Ni2+ | Escherichia coli | |
3.91 | - |
2',3'-cGMP | pH 7.2, 25°C, presence of Mn2+ | Escherichia coli | |
4.22 | - |
2',3'-cCMP | pH 7.2, 25°C, presence of Mn2+ | Escherichia coli | |
15.8 | - |
2',3'-cAMP | pH 7.2, 25°C, presence of Mn2+ | Escherichia coli |