Literature summary for 3.1.4.54 extracted from

Wang, J.; Ueda, N.
Biology of endocannabinoid synthesis system (2009), Prostaglandins Other Lipid Mediat., 89, 112-119.

Search for more literature for 3.1.4.54

Cloned(Commentary)

Cloned (Comment)	Organism
-	Mus musculus
-	Homo sapiens
-	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Canis lupus familiaris	16020	-
membrane	-	Rattus norvegicus	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	the enzyme belongs to the metallo-beta-lactamase family, presence of catalytically important zinc	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45596	-	45596, calculated from sequence	Homo sapiens
45723	-	x * 45723, calculated from sequence	Rattus norvegicus
45816	-	x * 45816, calculated from sequence	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-arachidonoylphosphatidylethanolamine + H2O	Canis lupus familiaris	the enzyme is involved in the biosynthesis of anandamide (N-arachidonoylethanolamine). NAPE-PLD is responsible for the conversion of N-acylphosphatidylethanolamines to N-acylethanolamines in vivo, but other enzyme(s) or pathway(s) are also involved in it, especially in the formation of polyunsaturated N-acylethanolamines, including anandamide. Unlike classical neurotransmitters and neuropeptides, endocannabinoids are not stored in vesicles in the cell, rather they are produced on demand from membrane phospholipids by a series of intracellular enzymes and released from cells, followed by immediate action as signaling molecules. Binding of endocannabinoids as well as cannabinoids to cannabinoid receptors results in the decrease in intracellular cyclic AMP level and the activation of mitogen-activated protein kinase through the coupled Gi/o proteins. The activation of cannabinoid receptors modulates ion channels through Gi/o proteins, leading to the activation of A-type and inwardly rectifying potassium channels and the inhibition of N-type and P/Q-type calcium channels. The endocannabinoid system is involved in a broad range of physiological functions, such as emotion, cardiovascular regulation, energy metabolism, and reproduction, and in a growing number of pathophysiological conditions	N-arachidonoylethanolamine + phosphatidic acid	N-arachidonoylethanolamine i.e. anandamide	?
N-arachidonoylphosphatidylethanolamine + H2O	Mus musculus	the enzyme is involved in the biosynthesis of anandamide (N-arachidonoylethanolamine). NAPE-PLD is responsible for the conversion of N-acylphosphatidylethanolamines to N-acylethanolamines in vivo, but other enzyme(s) or pathway(s) are also involved in it, especially in the formation of polyunsaturated N-acylethanolamines, including anandamide. Unlike classical neurotransmitters and neuropeptides, endocannabinoids are not stored in vesicles in the cell, rather they are produced on demand from membrane phospholipids by a series of intracellular enzymes and released from cells, followed by immediate action as signaling molecules. Binding of endocannabinoids as well as cannabinoids to cannabinoid receptors results in the decrease in intracellular cyclic AMP level and the activation of mitogen-activated protein kinase through the coupled Gi/o proteins. The activation of cannabinoid receptors modulates ion channels through Gi/o proteins, leading to the activation of A-type and inwardly rectifying potassium channels and the inhibition of N-type and P/Q-type calcium channels. The endocannabinoid system is involved in a broad range of physiological functions, such as emotion, cardiovascular regulation, energy metabolism, and reproduction, and in a growing number of pathophysiological conditions	N-arachidonoylethanolamine + phosphatidic acid	N-arachidonoylethanolamine i.e. anandamide	?
N-arachidonoylphosphatidylethanolamine + H2O	Homo sapiens	the enzyme is involved in the biosynthesis of anandamide (N-arachidonoylethanolamine). NAPE-PLD is responsible for the conversion of N-acylphosphatidylethanolamines to N-acylethanolamines in vivo, but other enzyme(s) or pathway(s) are also involved in it, especially in the formation of polyunsaturated N-acylethanolamines, including anandamide. Unlike classical neurotransmitters and neuropeptides, endocannabinoids are not stored in vesicles in the cell, rather they are produced on demand from membrane phospholipids by a series of intracellular enzymes and released from cells, followed by immediate action as signaling molecules. Binding of endocannabinoids as well as cannabinoids to cannabinoid receptors results in the decrease in intracellular cyclic AMP level and the activation of mitogen-activated protein kinase through the coupled Gi/o proteins. The activation of cannabinoid receptors modulates ion channels through Gi/o proteins, leading to the activation of A-type and inwardly rectifying potassium channels and the inhibition of N-type and P/Q-type calcium channels. The endocannabinoid system is involved in a broad range of physiological functions, such as emotion, cardiovascular regulation, energy metabolism, and reproduction, and in a growing number of pathophysiological conditions	N-arachidonoylethanolamine + phosphatidic acid	N-arachidonoylethanolamine i.e. anandamide	?
N-arachidonoylphosphatidylethanolamine + H2O	Rattus norvegicus	the enzyme is involved in the biosynthesis of anandamide (N-arachidonoylethanolamine). NAPE-PLD is responsible for the conversion of N-acylphosphatidylethanolamines to N-acylethanolamines in vivo, but other enzyme(s) or pathway(s) are also involved in it, especially in the formation of polyunsaturated N-acylethanolamines, including anandamide. Unlike classical neurotransmitters and neuropeptides, endocannabinoids are not stored in vesicles in the cell, rather they are produced on demand from membrane phospholipids by a series of intracellular enzymes and released from cells, followed by immediate action as signaling molecules. Binding of endocannabinoids as well as cannabinoids to cannabinoid receptors results in the decrease in intracellular cyclic AMP level and the activation of mitogen-activated protein kinase through the coupled Gi/o proteins. The activation of cannabinoid receptors modulates ion channels through Gi/o proteins, leading to the activation of A-type and inwardly rectifying potassium channels and the inhibition of N-type and P/Q-type calcium channels. The endocannabinoid system is involved in a broad range of physiological functions, such as emotion, cardiovascular regulation, energy metabolism, and reproduction, and in a growing number of pathophysiological conditions	N-arachidonoylethanolamine + phosphatidic acid	N-arachidonoylethanolamine i.e. anandamide	?

Organism

Organism	UniProt	Comment	Textmining
Canis lupus familiaris	-	-	-
Homo sapiens	Q6IQ20	-	-
Mus musculus	Q8BH82	-	-
Rattus norvegicus	Q769K2	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	the enzyme is not a subject to posttranslational modification	Mus musculus
additional information	the enzyme is not a subject to posttranslational modification	Homo sapiens
additional information	the enzyme is not a subject to posttranslational modification	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	lacks the ability to catalyze a transphosphatidylation	Canis lupus familiaris	?	-	?
additional information	lacks the ability to catalyze a transphosphatidylation	Rattus norvegicus	?	-	?
N-arachidonoylphosphatidylethanolamine + H2O	-	Canis lupus familiaris	N-arachidonoylethanolamine + phosphatidic acid	-	?
N-arachidonoylphosphatidylethanolamine + H2O	-	Mus musculus	N-arachidonoylethanolamine + phosphatidic acid	-	?
N-arachidonoylphosphatidylethanolamine + H2O	-	Homo sapiens	N-arachidonoylethanolamine + phosphatidic acid	-	?
N-arachidonoylphosphatidylethanolamine + H2O	-	Rattus norvegicus	N-arachidonoylethanolamine + phosphatidic acid	-	?
N-arachidonoylphosphatidylethanolamine + H2O	the enzyme is involved in the biosynthesis of anandamide (N-arachidonoylethanolamine). NAPE-PLD is responsible for the conversion of N-acylphosphatidylethanolamines to N-acylethanolamines in vivo, but other enzyme(s) or pathway(s) are also involved in it, especially in the formation of polyunsaturated N-acylethanolamines, including anandamide. Unlike classical neurotransmitters and neuropeptides, endocannabinoids are not stored in vesicles in the cell, rather they are produced on demand from membrane phospholipids by a series of intracellular enzymes and released from cells, followed by immediate action as signaling molecules. Binding of endocannabinoids as well as cannabinoids to cannabinoid receptors results in the decrease in intracellular cyclic AMP level and the activation of mitogen-activated protein kinase through the coupled Gi/o proteins. The activation of cannabinoid receptors modulates ion channels through Gi/o proteins, leading to the activation of A-type and inwardly rectifying potassium channels and the inhibition of N-type and P/Q-type calcium channels. The endocannabinoid system is involved in a broad range of physiological functions, such as emotion, cardiovascular regulation, energy metabolism, and reproduction, and in a growing number of pathophysiological conditions	Canis lupus familiaris	N-arachidonoylethanolamine + phosphatidic acid	N-arachidonoylethanolamine i.e. anandamide	?
N-arachidonoylphosphatidylethanolamine + H2O	the enzyme is involved in the biosynthesis of anandamide (N-arachidonoylethanolamine). NAPE-PLD is responsible for the conversion of N-acylphosphatidylethanolamines to N-acylethanolamines in vivo, but other enzyme(s) or pathway(s) are also involved in it, especially in the formation of polyunsaturated N-acylethanolamines, including anandamide. Unlike classical neurotransmitters and neuropeptides, endocannabinoids are not stored in vesicles in the cell, rather they are produced on demand from membrane phospholipids by a series of intracellular enzymes and released from cells, followed by immediate action as signaling molecules. Binding of endocannabinoids as well as cannabinoids to cannabinoid receptors results in the decrease in intracellular cyclic AMP level and the activation of mitogen-activated protein kinase through the coupled Gi/o proteins. The activation of cannabinoid receptors modulates ion channels through Gi/o proteins, leading to the activation of A-type and inwardly rectifying potassium channels and the inhibition of N-type and P/Q-type calcium channels. The endocannabinoid system is involved in a broad range of physiological functions, such as emotion, cardiovascular regulation, energy metabolism, and reproduction, and in a growing number of pathophysiological conditions	Mus musculus	N-arachidonoylethanolamine + phosphatidic acid	N-arachidonoylethanolamine i.e. anandamide	?
N-arachidonoylphosphatidylethanolamine + H2O	the enzyme is involved in the biosynthesis of anandamide (N-arachidonoylethanolamine). NAPE-PLD is responsible for the conversion of N-acylphosphatidylethanolamines to N-acylethanolamines in vivo, but other enzyme(s) or pathway(s) are also involved in it, especially in the formation of polyunsaturated N-acylethanolamines, including anandamide. Unlike classical neurotransmitters and neuropeptides, endocannabinoids are not stored in vesicles in the cell, rather they are produced on demand from membrane phospholipids by a series of intracellular enzymes and released from cells, followed by immediate action as signaling molecules. Binding of endocannabinoids as well as cannabinoids to cannabinoid receptors results in the decrease in intracellular cyclic AMP level and the activation of mitogen-activated protein kinase through the coupled Gi/o proteins. The activation of cannabinoid receptors modulates ion channels through Gi/o proteins, leading to the activation of A-type and inwardly rectifying potassium channels and the inhibition of N-type and P/Q-type calcium channels. The endocannabinoid system is involved in a broad range of physiological functions, such as emotion, cardiovascular regulation, energy metabolism, and reproduction, and in a growing number of pathophysiological conditions	Homo sapiens	N-arachidonoylethanolamine + phosphatidic acid	N-arachidonoylethanolamine i.e. anandamide	?
N-arachidonoylphosphatidylethanolamine + H2O	the enzyme is involved in the biosynthesis of anandamide (N-arachidonoylethanolamine). NAPE-PLD is responsible for the conversion of N-acylphosphatidylethanolamines to N-acylethanolamines in vivo, but other enzyme(s) or pathway(s) are also involved in it, especially in the formation of polyunsaturated N-acylethanolamines, including anandamide. Unlike classical neurotransmitters and neuropeptides, endocannabinoids are not stored in vesicles in the cell, rather they are produced on demand from membrane phospholipids by a series of intracellular enzymes and released from cells, followed by immediate action as signaling molecules. Binding of endocannabinoids as well as cannabinoids to cannabinoid receptors results in the decrease in intracellular cyclic AMP level and the activation of mitogen-activated protein kinase through the coupled Gi/o proteins. The activation of cannabinoid receptors modulates ion channels through Gi/o proteins, leading to the activation of A-type and inwardly rectifying potassium channels and the inhibition of N-type and P/Q-type calcium channels. The endocannabinoid system is involved in a broad range of physiological functions, such as emotion, cardiovascular regulation, energy metabolism, and reproduction, and in a growing number of pathophysiological conditions	Rattus norvegicus	N-arachidonoylethanolamine + phosphatidic acid	N-arachidonoylethanolamine i.e. anandamide	?

Subunits

Subunits	Comment	Organism
?	45596, calculated from sequence	Homo sapiens
?	x * 45723, calculated from sequence	Rattus norvegicus
?	x * 45816, calculated from sequence	Mus musculus

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Release 2023.1 (January 2023)