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Literature summary for 3.1.4.54 extracted from
- Radiochromatographic assay of N-acyl-phosphatidylethanolamine-specific phospholipase D activity (2005), Anal. Biochem., 339, 113-120.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 15-hydroxy-N-arachidonoylethanolamine | 0.005 mM, activation to 145% of the control | Mus musculus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-arachidonoylglycerol | 0.05 mM, 67% loss of activity | Mus musculus | |
| capsaicin | 0.005 mM, 20% inhibition | Mus musculus | |
| N-(4-hydroxy-2-methylphenyl)-arachidonoylamide | 0.005 mM, 82% inhibition | Mus musculus | |
| N-arachidonoylethanolamine | 0.05 mM, 37% loss of activity | Mus musculus | |
| N-oleoylethanolamine | 0.05 mM, 36% loss of activity | Mus musculus | |
| N-stearoylethanolamine | 0.005 mM, 14% loss of activity | Mus musculus | |
| noladin ether | 0.005 mM, 70% loss of activity | Mus musculus | |
| receptor agonist ACEA | 0.005 mM, 56% loss of activity | Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
N-arachidonoylphosphatidylethanolamine | pH 7.4, 37°C | Mus musculus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q8BH82 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Mus musculus | - |
| cerebellum | - |
Mus musculus | - |
| cerebral cortex | - |
Mus musculus | - |
| corpus striatum | - |
Mus musculus | - |
| liver | highest specific acitivity | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000193 | - |
pH 7.4, 37°C, liver | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-arachidonoylphosphatidylethanolamine + H2O | - |
Mus musculus | N-arachidonoylethanolamine + phosphatidic acid | N-arachidonoylethanolamine i.e. anandamide | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| N-acyl-phosphatidylethanolamine-specific phospholipase D | - |
Mus musculus |
| NAPE-PLD | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mus musculus |
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Release 2023.1 (January 2023)
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