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Literature summary for 3.1.4.53 extracted from

  • Byrne, A.M.; Elliott, C.; Hoffmann, R.; Baillie, G.S.
    The activity of cAMP-phosphodiesterase 4D7 (PDE4D7) is regulated by protein kinase A-dependent phosphorylation within its unique N-terminus (2015), FEBS Lett., 589, 750-755 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
S42A the mutant with 170% activity compared to the wild type enzyme is not phosphorylated at serine 42 Homo sapiens
S42D the mutant with increased activity compared to the wild type enzyme is not phosphorylated at serine 42 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3',5'-cAMP + H2O Homo sapiens
-
5'-AMP
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q08499
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the enzyme is phosphorylated and activated by protein kinase A within upstream conserved region 1 and inhibited by phosphorylation at serine 42 within the N-terminal region Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3',5'-cAMP + H2O
-
Homo sapiens 5'-AMP
-
?

Synonyms

Synonyms Comment Organism
cAMP-phosphodiesterase 4D7
-
Homo sapiens
cyclic AMP phosphodiesterase type 4
-
Homo sapiens
PDE4D7
-
Homo sapiens

Expression

Organism Comment Expression
Homo sapiens isoform PDE4D7 doubles in activity after forskolin treatment up

General Information

General Information Comment Organism
physiological function isoform PDE4D7 is important in prostate cancer progression and ischemic stroke Homo sapiens