Cloned (Comment) | Organism |
---|---|
the DdPDE4 catalytic domain (amino acids 264-771) is expressed in vegetative AX2 cells | Dictyostelium discoideum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme knock-out strains show normal aggregation but impaired development from the mound stage on | Dictyostelium discoideum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0096 | - |
adenosine 3',5'-cyclic phosphate | - |
Dictyostelium discoideum | |
0.01 | - |
cAMP | pH 7.0, 37°C | Dictyostelium discoideum | |
0.01 | - |
3',5'-cAMP | 37°C, pH 7.0 | Dictyostelium discoideum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Dictyostelium discoideum | 16020 | - |
membrane | membrane-bound enzyme with an extracellular domain | Dictyostelium discoideum | 16020 | - |
membrane | DdPDE4 is a unique membrane-bound phosphodiesterase with an extracellular catalytic domain regulating intercellular cAMP during multicellular development | Dictyostelium discoideum | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3',5'-cAMP + H2O | Dictyostelium discoideum | DdPDE4 regulates intercellular cAMP during multicellular development | 5'-AMP | - |
? | |
adenosine 3',5'-cyclic phosphate + H2O | Dictyostelium discoideum | DdPDE4 is a unique membrane-bound phosphodiesterase with an extracellular catalytic domain regulating intercellular cAMP during multicellular development | adenosine 5'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | - |
- |
- |
Dictyostelium discoideum | Q86H13 | - |
- |
Dictyostelium discoideum | Q86H13 | isoform PDE4, enzyme is expressed during single cell stages and expression increases after 9 h of starvation | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | protein sequence predicts a leader sequence, two transmembrane segments, and an extracellular catalytic domain | Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3',5'-cAMP + H2O | - |
Dictyostelium discoideum | 5'-AMP | - |
? | |
3',5'-cAMP + H2O | DdPDE4 regulates intercellular cAMP during multicellular development | Dictyostelium discoideum | 5'-AMP | - |
? | |
adenosine 3',5'-cyclic phosphate + H2O | - |
Dictyostelium discoideum | adenosine 5'-phosphate | - |
? | |
adenosine 3',5'-cyclic phosphate + H2O | DdPDE4 is a unique membrane-bound phosphodiesterase with an extracellular catalytic domain regulating intercellular cAMP during multicellular development | Dictyostelium discoideum | adenosine 5'-phosphate | - |
? | |
additional information | cGMP is hydrolyzed at least 100fold more slowly than cAMP | Dictyostelium discoideum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DdPDE4 | - |
Dictyostelium discoideum |