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Literature summary for 3.1.4.53 extracted from
- Refolding and Purification of Recombinant Human PDE7A: Expressed in Escherichia coli as Inclusion Bodies (2002), Protein Expr. Purif., 25, 138-148.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cGMP | 5% inhibition at 0.1 mM | Homo sapiens |  |
| erythro-9-(2-hydroxy-3-nonyl)adenine | 7.4% inhibition at 0.1 mM | Homo sapiens | |
| Milrinone | 42% inhibition at 0.1 mM | Homo sapiens | |
| rolipram | 18% inhibition at 0.1 mM | Homo sapiens | |
| zaprinast | 10% inhibition at 0.05 mM | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
cAMP | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38800 | - |
x * 39000, SDS-PAGE, x * 38800, deduced from gene sequence, both N-terminally truncated enzyme | Homo sapiens |
| 39000 | - |
x * 39000, SDS-PAGE, x * 38800, deduced from gene sequence, both N-terminally truncated enzyme | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
PDE7A, recombinant protein, N-terminally truncated | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from inclusion bodies | Homo sapiens |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| refolding to active enzyme from inclusion bodies requires high concentrations of arginine hydrochloride, ethylene glycol, and magnesium chloride at pH 8.5 | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cAMP + H2O | - |
Homo sapiens | AMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 39000, SDS-PAGE, x * 38800, deduced from gene sequence, both N-terminally truncated enzyme | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
cAMP | - |
Homo sapiens | |
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