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Literature summary for 3.1.4.52 extracted from

  • Miner, K.D.; Kurtz, D.M.
    Active site metal occupancy and cyclic di-GMP phosphodiesterase activity of Thermotoga maritima HD-GYP (2016), Biochemistry, 55, 970-979 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.52

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21pLysE (DE3) cells Thermotoga maritima

Protein Variants

Protein Variants Comment Organism
D289A inactive Thermotoga maritima
E169A the mutant enzyme is active with 3',5'-di-cGMP Thermotoga maritima
H173A inactive Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Iron accommodates di- and triiron active site Thermotoga maritima
Mn2+ contains Mn2+ Thermotoga maritima
additional information the enzyme can accommodate both di- and trimetal active sites. An as-isolated iron-containing TM0186 has an oxo/carboxylato-bridged diferric site, and the reduced diferrous form is necessary and sufficient to catalyze conversion of cyclic di-GMP to 5'-phosphoguanylyl(3',5')guanosine. Similar cyclic di-GMP phosphodiesterase activities are obtained with divalent iron or manganese. A Glu residue conserved in a subset of homologous enzymes is required for formation of the trimetal site and can also serve as a labile ligand to the dimetal site Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 gel filtration Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3',5'-di-cGMP + H2O Thermotoga maritima
-
 5'-phosphoguanylyl-(3'->5')guanosine
-
 ?
3',5'-di-cGMP + H2O Thermotoga maritima DSM 3109
-
 5'-phosphoguanylyl-(3'->5')guanosine
-
 ?
3',5'-di-cGMP + H2O Thermotoga maritima ATCC 43589
-
 5'-phosphoguanylyl-(3'->5')guanosine
-
 ?
5'-phosphoguanylyl-(3'->5')guanosine + H2O Thermotoga maritima
-
 2 5'-GMP
-
 ?
5'-phosphoguanylyl-(3'->5')guanosine + H2O Thermotoga maritima DSM 3109
-
 2 5'-GMP
-
 ?
5'-phosphoguanylyl-(3'->5')guanosine + H2O Thermotoga maritima ATCC 43589
-
 2 5'-GMP
-
 ?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9WY30
-
-
Thermotoga maritima ATCC 43589 Q9WY30
-
-
Thermotoga maritima DSM 3109 Q9WY30
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose resin column chromatography Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3',5'-di-cGMP + H2O
-
 Thermotoga maritima 5'-phosphoguanylyl-(3'->5')guanosine
-
 ?
3',5'-di-cGMP + H2O
-
 Thermotoga maritima DSM 3109 5'-phosphoguanylyl-(3'->5')guanosine
-
 ?
3',5'-di-cGMP + H2O
-
 Thermotoga maritima ATCC 43589 5'-phosphoguanylyl-(3'->5')guanosine
-
 ?
5'-phosphoguanylyl-(3'->5')guanosine + H2O
-
 Thermotoga maritima 2 5'-GMP
-
 ?
5'-phosphoguanylyl-(3'->5')guanosine + H2O
-
 Thermotoga maritima DSM 3109 2 5'-GMP
-
 ?
5'-phosphoguanylyl-(3'->5')guanosine + H2O
-
 Thermotoga maritima ATCC 43589 2 5'-GMP
-
 ?

Subunits

Subunits Comment Organism
? x * 90000, His6- and maltose binding protein-tagged enzyme, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
c-di-GMP phosphodiesterase
-
 Thermotoga maritima
cyclic di-GMP phosphodiesterase
-
 Thermotoga maritima
HD-GYP
-
 Thermotoga maritima
TM0186
-
 Thermotoga maritima
TM_0186
-
 Thermotoga maritima