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Literature summary for 3.1.4.52 extracted from
- The EAL domain protein VieA is a cyclic diguanylate phosphodiesterase (2005), J. Biol. Chem., 280, 33324-33330.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| KCl | highest catalytic activity in presence of 25 mM KCl | Vibrio cholerae serotype O1 |  |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E170A | mutation in metal binding site, loss of activity | Vibrio cholerae serotype O1 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | strong | Vibrio cholerae serotype O1 | |
| Zn2+ | strong | Vibrio cholerae serotype O1 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00006 | - |
cyclic di-3',5'-guanylate | 23°C, pH 7.0 | Vibrio cholerae serotype O1 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | or Mn2+, required, with Mg2+ being more efficient. Enzyme activity increases with with increasing Mg2+ concentration up to 25 mM MgCl2 | Vibrio cholerae serotype O1 | |
| Mn2+ | or Mg2+, required, with Mg2+ being more efficient | Vibrio cholerae serotype O1 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio cholerae serotype O1 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyclic di-3',5'-guanylate + H2O | - |
Vibrio cholerae serotype O1 | 5'-phosphoguanylyl(3'-5')guanosine | - |
? | |
| additional information | no substrate: cyclic guanosine monophosphate | Vibrio cholerae serotype O1 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| VieA | - |
Vibrio cholerae serotype O1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | 30 | - |
Vibrio cholerae serotype O1 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 52 | - |
loss of activity above | Vibrio cholerae serotype O1 |
| 65 | - |
no residual activity | Vibrio cholerae serotype O1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Vibrio cholerae serotype O1 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | no significant changes in reaction rates within this range | Vibrio cholerae serotype O1 |
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Release 2023.1 (January 2023)
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