Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli with His-tag | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of gene PA4367 results in severe defect in swarming motility and a hyperbiofilm phenotype. Mutant exhibits increased cellular pools of cyclic di-3,5-guanylate, increased synthesis of a polysaccharide produced by the pel locus and decreased flagellar reversals. GGDQF and EAL domains of BifA are both required for complementation of the mutant | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | inner membrane | Pseudomonas aeruginosa | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
x * 78000, calculated | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
strain PA14, gene PA4367 encodes a protein with an EAL domain associated with cyclic di-3'5'-guanylate phosphodiesterase activity, and a DDGEF domain associated with cyclic di-di-3'5'-guanylate cyclase activity | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | purified enzyme shows phosphodiesterase activity, but no diguanylate cyclase activity | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, calculated | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
BifA | - |
Pseudomonas aeruginosa |
PA4367 | - |
Pseudomonas aeruginosa |