BRENDA - Enzyme Database
show all sequences of 3.1.4.49

Characterization and partial purification of a novel mannosylphosphodolichol phosphodiesterase from chicken liver microsomes

Tomita, Y.; Motokawa, Y.; Eur. J. Biochem. 170, 363-368 (1987)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
requirement, or other SH-reducing agents
Gallus gallus
Emulgen 909
requirement, optimum concentration: 0.4% v/v
Gallus gallus
additional information
no requirement for any phospholipid
Gallus gallus
SH-reducing agents
e.g. 2-mercaptoethanol, absolute requirement
Gallus gallus
General Stability
General Stability
Organism
a heat-stable factor, precipitable by trichloroacetic acid, insoluble in lipid solvents, stabilizes, separation from this factor by ion-exchange chromatography inactivates irreversibly
Gallus gallus
Inhibitors
Inhibitors
Commentary
Organism
Structure
bis(4-nitrophenyl)phosphate
1 mM, competitive inhibition
Gallus gallus
dolichol
0.1 mM, strong, competitive inhibition
Gallus gallus
dolichyl phosphate
0.1 mM, strong, competitive inhibition
Gallus gallus
additional information
not inhibited by D-mannose, mannose 1-phosphate, GDP, GMP, ADP, AMP, phosphate, diphosphate GDPmannose, UDP-N-acetylglucosamine, UDPglucose, UDPglucuronic acid, 4-nitrophenyl phosphate, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside or 4-nitrophenyl-beta-D-glucopyranoside
Gallus gallus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00043
-
Mannosylphosphodolichol
pH 5.3, 37°C
Gallus gallus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
predominantly
Gallus gallus
-
-
microsome
-
Rattus norvegicus
-
-
mitochondrion
-
Gallus gallus
5739
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
CaCl2
greatly stimulates, optimum concentration: 2 mM
Gallus gallus
additional information
not activated by other divalent cations than Ca2+
Gallus gallus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
mannosylphosphodolichol + H2O
Gallus gallus
enzyme may be capable of degrading Dol-P-Man in vivo
D-mannose + dolichyl phosphate
-
Gallus gallus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gallus gallus
-
-
-
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
partial, 57.9fold, solubilized with 1% v/v Emulgen 909
Gallus gallus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Gallus gallus
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00000011
-
pH 6.3
Rattus norvegicus
0.000631
-
pH 5.3, 37°C
Gallus gallus
Storage Stability
Storage Stability
Organism
0°C, in the presence of 2-mercaptoethanol and stabilizing factor, 1 month, stable
Gallus gallus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
mannosylphosphodolichol + H2O
-
646471
Rattus norvegicus
D-mannose + dolichyl phosphate
-
646471
Rattus norvegicus
?
mannosylphosphodolichol + H2O
highly specific for Dol-P-Man
646471
Gallus gallus
D-mannose + dolichyl phosphate
-
646471
Gallus gallus
?
mannosylphosphodolichol + H2O
enzyme may be capable of degrading Dol-P-Man in vivo
646471
Gallus gallus
D-mannose + dolichyl phosphate
-
646471
Gallus gallus
?
additional information
not: N-acetylglucosaminyldiphosphodolichol, glucosylphosphodolichol or mannose 1-phosphate, artificial substrates for acid phosphatase, acid phosphodiesterase, mannosidase or glucosidase
646471
Gallus gallus
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Gallus gallus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3
-
acetate buffer
Gallus gallus
6.3
-
assay at
Rattus norvegicus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0125
-
dolichyl phosphate
pH 5.3, 37°C
Gallus gallus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
requirement, or other SH-reducing agents
Gallus gallus
Emulgen 909
requirement, optimum concentration: 0.4% v/v
Gallus gallus
additional information
no requirement for any phospholipid
Gallus gallus
SH-reducing agents
e.g. 2-mercaptoethanol, absolute requirement
Gallus gallus
General Stability (protein specific)
General Stability
Organism
a heat-stable factor, precipitable by trichloroacetic acid, insoluble in lipid solvents, stabilizes, separation from this factor by ion-exchange chromatography inactivates irreversibly
Gallus gallus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
bis(4-nitrophenyl)phosphate
1 mM, competitive inhibition
Gallus gallus
dolichol
0.1 mM, strong, competitive inhibition
Gallus gallus
dolichyl phosphate
0.1 mM, strong, competitive inhibition
Gallus gallus
additional information
not inhibited by D-mannose, mannose 1-phosphate, GDP, GMP, ADP, AMP, phosphate, diphosphate GDPmannose, UDP-N-acetylglucosamine, UDPglucose, UDPglucuronic acid, 4-nitrophenyl phosphate, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside or 4-nitrophenyl-beta-D-glucopyranoside
Gallus gallus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0125
-
dolichyl phosphate
pH 5.3, 37°C
Gallus gallus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00043
-
Mannosylphosphodolichol
pH 5.3, 37°C
Gallus gallus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
predominantly
Gallus gallus
-
-
microsome
-
Rattus norvegicus
-
-
mitochondrion
-
Gallus gallus
5739
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
CaCl2
greatly stimulates, optimum concentration: 2 mM
Gallus gallus
additional information
not activated by other divalent cations than Ca2+
Gallus gallus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
mannosylphosphodolichol + H2O
Gallus gallus
enzyme may be capable of degrading Dol-P-Man in vivo
D-mannose + dolichyl phosphate
-
Gallus gallus
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial, 57.9fold, solubilized with 1% v/v Emulgen 909
Gallus gallus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Gallus gallus
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00000011
-
pH 6.3
Rattus norvegicus
0.000631
-
pH 5.3, 37°C
Gallus gallus
Storage Stability (protein specific)
Storage Stability
Organism
0°C, in the presence of 2-mercaptoethanol and stabilizing factor, 1 month, stable
Gallus gallus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
mannosylphosphodolichol + H2O
-
646471
Rattus norvegicus
D-mannose + dolichyl phosphate
-
646471
Rattus norvegicus
?
mannosylphosphodolichol + H2O
highly specific for Dol-P-Man
646471
Gallus gallus
D-mannose + dolichyl phosphate
-
646471
Gallus gallus
?
mannosylphosphodolichol + H2O
enzyme may be capable of degrading Dol-P-Man in vivo
646471
Gallus gallus
D-mannose + dolichyl phosphate
-
646471
Gallus gallus
?
additional information
not: N-acetylglucosaminyldiphosphodolichol, glucosylphosphodolichol or mannose 1-phosphate, artificial substrates for acid phosphatase, acid phosphodiesterase, mannosidase or glucosidase
646471
Gallus gallus
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Gallus gallus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3
-
acetate buffer
Gallus gallus
6.3
-
assay at
Rattus norvegicus
Other publictions for EC 3.1.4.49
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
646471
Tomita
Characterization and partial p ...
Gallus gallus, Rattus norvegicus
Eur. J. Biochem.
170
363-368
1987
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