BRENDA - Enzyme Database
show all sequences of 3.1.4.48

Synthesis and degradation of dolichyl phosphoryl glucose by the cellular slime mold, Dictyostelium discoideum

Crean, E.V.; Biochim. Biophys. Acta 792, 149-157 (1984)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Triton X-100
-
Dictyostelium discoideum
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
not markedly inhibited by alpha-or beta-glucosides or by nucleotides, exhibits a very low capacity to transfer glucose to endogenous oligosaccharide-lipid or protein acceptors in presence of EDTA, MgCl2 or CaCl2
Dictyostelium discoideum
PCMB
completely inhibited by 1 mM
Dictyostelium discoideum
UDP-GlcUA
most effective inhibitor, 0.025-0.050 mM, 50% inhibition
Dictyostelium discoideum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
less than 10% of the activation with Mn2+
Dictyostelium discoideum
Mg2+
less than 10% of the activation with Mn2+
Dictyostelium discoideum
Mn2+
dependent on, can activate in presence of potassium phosphate, Km for MnCl2 less than 1 mM
Dictyostelium discoideum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Dictyostelium discoideum
-
cellular slime mold, strain NC-4
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dolichyl beta-D-glucosyl phosphate + H2O
-
646470
Dictyostelium discoideum
dolichyl phosphate + D-glucose
-
646470
Dictyostelium discoideum
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
27
30
-
Dictyostelium discoideum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
6
-
Dictyostelium discoideum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Triton X-100
-
Dictyostelium discoideum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
not markedly inhibited by alpha-or beta-glucosides or by nucleotides, exhibits a very low capacity to transfer glucose to endogenous oligosaccharide-lipid or protein acceptors in presence of EDTA, MgCl2 or CaCl2
Dictyostelium discoideum
PCMB
completely inhibited by 1 mM
Dictyostelium discoideum
UDP-GlcUA
most effective inhibitor, 0.025-0.050 mM, 50% inhibition
Dictyostelium discoideum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
less than 10% of the activation with Mn2+
Dictyostelium discoideum
Mg2+
less than 10% of the activation with Mn2+
Dictyostelium discoideum
Mn2+
dependent on, can activate in presence of potassium phosphate, Km for MnCl2 less than 1 mM
Dictyostelium discoideum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dolichyl beta-D-glucosyl phosphate + H2O
-
646470
Dictyostelium discoideum
dolichyl phosphate + D-glucose
-
646470
Dictyostelium discoideum
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
27
30
-
Dictyostelium discoideum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
6
-
Dictyostelium discoideum
Other publictions for EC 3.1.4.48
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
646470
Crean
-
Synthesis and degradation of d ...
Dictyostelium discoideum
Biochim. Biophys. Acta
792
149-157
1984
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