Literature summary for 3.1.4.46 extracted from

Hadler, K.S.; Tanifum, E.A.; Yip, S.H.; Mitic, N.; Guddat, L.W.; Jackson, C.J.; Gahan, L.R.; Nguyen, K.; Carr, P.D.; Ollis, D.L.; Hengge, A.C.; Larrabee, J.A.; Schenk, G.
Substrate-promoted formation of a catalytically competent binuclear center and regulation of reactivity in a glycerophosphodiesterase from Enterobacter aerogenes (2008), J. Am. Chem. Soc., 130, 14129-14138.

Search for more literature for 3.1.4.46

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli DH5alpha	Klebsiella aerogenes

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Klebsiella aerogenes

Protein Variants

Protein Variants	Comment	Organism
N80A	ligand of the metal ion	Klebsiella aerogenes
N80D	ligand of the metal ion	Klebsiella aerogenes

Inhibitors

Inhibitors	Comment	Organism	Structure
p-nitrophenyl phosphate	-	Klebsiella aerogenes
phosphate	hydrolysis of p-nitrophenyl phosphate, competitive	Klebsiella aerogenes

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.12	-	p-nitrophenyl phosphate	wild-type protein, pH 7.0, 1 mM CoCl2	Klebsiella aerogenes
39	-	p-nitrophenyl phosphate	N80D mutant protein, pH 7.0, 1 mM CoCl2	Klebsiella aerogenes
49	-	p-nitrophenyl phosphate	N80A mutant protein, pH 7.0, 1 mM CoCl2	Klebsiella aerogenes

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	a Co(II)-substituted enzyme is used	Klebsiella aerogenes

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31000	-	x * 31000, SDS-PAGE	Klebsiella aerogenes

Organism

Organism	UniProt	Comment	Textmining
Klebsiella aerogenes	Q6XBH1	-	-

Purification (Commentary)

Purification (Comment)	Organism
ion-exchange chromatography (DEAE), hydrophobic interaction chromatography (Phenyl Sepharose), gel filtration	Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
bis(p-nitrophenyl) phosphate + H2O	-	Klebsiella aerogenes	p-nitrophenyl phosphate + p-nitrophenol	-	?
ethyl p-nitrophenyl phosphate + H2O	-	Klebsiella aerogenes	p-nitrophenol + ethyl phosphate	-	?
p-nitrophenyl phosphate + H2O	-	Klebsiella aerogenes	p-nitrophenol + phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 31000, SDS-PAGE	Klebsiella aerogenes

Synonyms

Synonyms	Comment	Organism
glycerophosphodiesterase	-	Klebsiella aerogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.24	-	p-nitrophenyl phosphate	N80D mutant protein, pH 7.0, 1 mM CoCl2	Klebsiella aerogenes
1.89	-	p-nitrophenyl phosphate	wild-type protein, pH 7.0, 1 mM CoCl2	Klebsiella aerogenes
6.4	-	p-nitrophenyl phosphate	N80A mutant protein, pH 7.0, 1 mM CoCl2	Klebsiella aerogenes

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.078	-	p-nitrophenyl phosphate	-	Klebsiella aerogenes

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.006	-	p-nitrophenyl phosphate	N80D mutant protein, pH 7.0, 1 mM CoCl2	Klebsiella aerogenes
0.13	-	p-nitrophenyl phosphate	N80A mutant protein, pH 7.0, 1 mM CoCl2	Klebsiella aerogenes
16.4	-	p-nitrophenyl phosphate	wild-type protein, pH 7.0, 1 mM CoCl2	Klebsiella aerogenes

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)