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Literature summary for 3.1.4.45 extracted from

  • Varki, A.; Kornfeld, S.
    Purification and characterization of rat liver alpha-N-acetylglucosaminyl phosphodiesterase (1981), J. Biol. Chem., 256, 9937-9943.
    View publication on PubMed
Search for more literature for 3.1.4.45

General Stability

General Stability Organism
loss of activity by repeated freezing and thawing, or dialysis Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
mannose-6-phosphate
-
 Rattus sp.
N-acetylglucosamine
-
 Rattus sp.
N-acetylglucosamine phosphate
-
 Rattus sp.
PO43-
-
 Rattus sp.
UDP-GlcNAc
-
 Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose
-
 Rattus sp.
1.3
-
 UDP-N-acetylglucosamine
-
 Rattus sp.
1.6
-
 N-acetylglucosamine 1-phosphate
-
 Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane cis part Rattus sp. 139
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information unaffected by divalent cation Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O Rattus sp.
-
 N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose
-
 ?
additional information Rattus sp. acts on a variety of compounds with N-acetyl-D-glucosamine alpha-linked to phosphate group, including biosynthetic intermediates of the high mannose oligosaccharide components of some lysosomal enzymes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining

Storage Stability

Storage Stability Organism
27% loss of activity after 120 days at -80°C Rattus sp.
50% loss of activity after 120 days at 4°C Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O
-
 Rattus sp. N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose
-
 ?
methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose + H2O
-
 Rattus sp. N-acetyl-D-glucosamine + methyl alpha-D-mannose 6-phosphate
-
 ?
additional information acts on a variety of compounds with N-acetyl-D-glucosamine alpha-linked to phosphate group, including biosynthetic intermediates of the high mannose oligosaccharide components of some lysosomal enzymes Rattus sp. ?
-
 ?
N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Rattus sp. N-acetyl-D-glucosamine + phosphate
-
 ?
UDP-N-acetyl-alpha-D-glucosamine + H2O
-
 Rattus sp. N-acetyl-D-glucosamine + UDP
-
 ?
UDP-N-acetyl-D-glucosamine + H2O
-
 Rattus sp. UDP + N-acetyl-D-glucosamine
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
 Rattus sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 10
-
 Rattus sp.