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Literature summary for 3.1.4.42 extracted from
- rac-Glycerol 1:2-cyclic phosphate 2-phosphodiesterase, a new soluble phosphodiesterase of mammalian tissues (1978), Biochem. J., 173, 579-589.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | stimulation | Sus scrofa |  |
| cysteine | stimulation | Sus scrofa | |
| dithiothreitol | stimulation | Sus scrofa | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2'-AMP | 9 mM, 75% inhibition | Sus scrofa | |
| 2-naphthyl phosphate | 0.45 mM, 64% inhibition | Sus scrofa | |
| 3'-AMP | 9 mM, 68% inhibition | Sus scrofa | |
| 4-nitrophenyl phosphate | 5.5 mM, 90% inhibition | Sus scrofa | |
| 5'-AMP | 9 mM, 76% inhibition | Sus scrofa | |
| ATP | 9 mM, 68% inhibition | Sus scrofa | |
| cetyl phosphate | 0.45 mM, 29% inhibition | Sus scrofa | |
| dibutyryl glycerol phosphate | 0.45 mM, 40% inhibition | Sus scrofa | |
| ethyl phosphate | 0.25 mM, 34% inhibition | Sus scrofa | |
| glycerol 1-phosphate | 5 mM, 16% inhibition | Sus scrofa | |
| methyl phosphate | 0.25 mM, 34% inhibition | Sus scrofa | |
| Phenyl phosphate | 0.45 mM, 68% inhibition | Sus scrofa | |
| phosphatidic acid | 0.45 mM, 43% inhibition | Sus scrofa | |
| PO43- | - |
Sus scrofa | |
| pyridoxal 5'-phosphate | 0.25 mM, 14% inhibition | Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.2 | - |
glycerol 1,2-cyclic phosphate | - |
Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Cavia porcellus | 5737 | - |
| cytoplasm | - |
Mus musculus | 5737 | - |
| cytoplasm | - |
Rattus norvegicus | 5737 | - |
| cytoplasm | - |
Sus scrofa | 5737 | - |
| cytoplasm | - |
Ovis aries | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | stimulates | Sus scrofa | |
| additional information | no requirement for Ca2+ and Mg2+ | Sus scrofa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35500 | - |
gel filtration | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
| Mus musculus | - |
- |
- |
| Ovis aries | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cerebral hemisphere | low activity | Cavia porcellus | - |
| cerebral hemisphere | low activity | Rattus norvegicus | - |
| intestinal mucosa | - |
Rattus norvegicus | - |
| kidney | - |
Cavia porcellus | - |
| kidney | - |
Mus musculus | - |
| kidney | - |
Rattus norvegicus | - |
| kidney | - |
Sus scrofa | - |
| kidney | - |
Ovis aries | - |
| liver | - |
Cavia porcellus | - |
| liver | - |
Mus musculus | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Sus scrofa | - |
| liver | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 322 | - |
- |
Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2',3'-cyclic AMP + H2O | - |
Sus scrofa | 3'-AMP + 2'-AMP | 69-72% 3'-AMP and 28-31% 2'-AMP | ? | |
| 3',5'-cyclic AMP + H2O | - |
Sus scrofa | 5'-AMP | - |
? | |
| 3',5'-cyclic GMP + H2O | very low activity | Sus scrofa | ? | - |
? | |
| bis-(4-nitrophenyl) phosphate + H2O | very low activity | Sus scrofa | ? | - |
? | |
| butyrylglycerol cyclic phosphate + H2O | - |
Sus scrofa | butyrylglycerol phosphate | - |
? | |
| glycerol 1,2-cyclic phosphate + H2O | racemic substrate | Cavia porcellus | glycerol phosphate | - |
? | |
| glycerol 1,2-cyclic phosphate + H2O | racemic substrate | Mus musculus | glycerol phosphate | - |
? | |
| glycerol 1,2-cyclic phosphate + H2O | racemic substrate | Rattus norvegicus | glycerol phosphate | - |
? | |
| glycerol 1,2-cyclic phosphate + H2O | racemic substrate | Sus scrofa | glycerol phosphate | - |
? | |
| glycerol 1,2-cyclic phosphate + H2O | racemic substrate | Ovis aries | glycerol phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
assay at | Cavia porcellus |
| 38 | - |
assay at | Mus musculus |
| 38 | - |
assay at | Rattus norvegicus |
| 38 | - |
assay at | Sus scrofa |
| 38 | - |
assay at | Ovis aries |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
beta,beta-dimethylglutarate buffer | Sus scrofa |
| 6.9 | 7.5 | - |
Sus scrofa |
| 7.4 | - |
imidazole /HCl buffer | Sus scrofa |
| 7.5 | - |
glycylglycine buffer | Sus scrofa |
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