Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.41 extracted from

  • Gellings, P.S.; McGee, D.J.
    Arcanobacterium haemolyticum phospholipase D enzymatic activity promotes the hemolytic activity of the cholesterol-dependent cytolysin arcanolysin (2018), Toxins, 10, E213 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10F' cells Arcanobacterium haemolyticum

Protein Variants

Protein Variants Comment Organism
H49A the mutant shows reduced activity compared to the wild type enzyme Arcanobacterium haemolyticum
N68A the mutant shows reduced activity compared to the wild type enzyme Arcanobacterium haemolyticum
P43A the mutant shows reduced activity compared to the wild type enzyme Arcanobacterium haemolyticum
P43A/H49A the mutant shows strongly reduced activity compared to the wild type enzyme Arcanobacterium haemolyticum
P43A/Y45A the mutant shows strongly reduced activity compared to the wild type enzyme Arcanobacterium haemolyticum
T275A the mutant shows reduced activity compared to the wild type enzyme Arcanobacterium haemolyticum
Y45A the mutant shows reduced activity compared to the wild type enzyme Arcanobacterium haemolyticum
Y45A/H49A the mutant shows strongly reduced activity compared to the wild type enzyme Arcanobacterium haemolyticum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphingomyelin + H2O Arcanobacterium haemolyticum
-
ceramide phosphate + choline
-
?
sphingomyelin + H2O Arcanobacterium haemolyticum ATCC 9345
-
ceramide phosphate + choline
-
?

Organism

Organism UniProt Comment Textmining
Arcanobacterium haemolyticum C7SZ47
-
-
Arcanobacterium haemolyticum ATCC 9345 C7SZ47
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Arcanobacterium haemolyticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphingomyelin + H2O
-
Arcanobacterium haemolyticum ceramide phosphate + choline
-
?
sphingomyelin + H2O
-
Arcanobacterium haemolyticum ATCC 9345 ceramide phosphate + choline
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Arcanobacterium haemolyticum

Synonyms

Synonyms Comment Organism
PLD
-
Arcanobacterium haemolyticum
sphingomyelin-specific phospholipase D
-
Arcanobacterium haemolyticum

General Information

General Information Comment Organism
physiological function the enzyme activity is necessary to promote arcanolysin-mediated hemolysis of erythrocytes in both time- and concentration-dependent manners Arcanobacterium haemolyticum