Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10F' cells | Arcanobacterium haemolyticum |
Protein Variants | Comment | Organism |
---|---|---|
H49A | the mutant shows reduced activity compared to the wild type enzyme | Arcanobacterium haemolyticum |
N68A | the mutant shows reduced activity compared to the wild type enzyme | Arcanobacterium haemolyticum |
P43A | the mutant shows reduced activity compared to the wild type enzyme | Arcanobacterium haemolyticum |
P43A/H49A | the mutant shows strongly reduced activity compared to the wild type enzyme | Arcanobacterium haemolyticum |
P43A/Y45A | the mutant shows strongly reduced activity compared to the wild type enzyme | Arcanobacterium haemolyticum |
T275A | the mutant shows reduced activity compared to the wild type enzyme | Arcanobacterium haemolyticum |
Y45A | the mutant shows reduced activity compared to the wild type enzyme | Arcanobacterium haemolyticum |
Y45A/H49A | the mutant shows strongly reduced activity compared to the wild type enzyme | Arcanobacterium haemolyticum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Arcanobacterium haemolyticum | - |
ceramide phosphate + choline | - |
? | |
sphingomyelin + H2O | Arcanobacterium haemolyticum ATCC 9345 | - |
ceramide phosphate + choline | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arcanobacterium haemolyticum | C7SZ47 | - |
- |
Arcanobacterium haemolyticum ATCC 9345 | C7SZ47 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Arcanobacterium haemolyticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | - |
Arcanobacterium haemolyticum | ceramide phosphate + choline | - |
? | |
sphingomyelin + H2O | - |
Arcanobacterium haemolyticum ATCC 9345 | ceramide phosphate + choline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Arcanobacterium haemolyticum |
Synonyms | Comment | Organism |
---|---|---|
PLD | - |
Arcanobacterium haemolyticum |
sphingomyelin-specific phospholipase D | - |
Arcanobacterium haemolyticum |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme activity is necessary to promote arcanolysin-mediated hemolysis of erythrocytes in both time- and concentration-dependent manners | Arcanobacterium haemolyticum |