Cloned (Comment) | Organism |
---|---|
recombinant expression of N-terminally His-tagged recombinant enzyme in Escherichia coli strain BL21(lDE3) | Loxosceles reclusa |
recombinant expression of N-terminally His-tagged recombinant enzyme in Escherichia coli strain BL21(lDE3) | Loxosceles laeta |
recombinant expression of N-terminally His-tagged recombinant enzyme in Escherichia coli strain BL21(lDE3) | Loxosceles arizonica |
Protein Variants | Comment | Organism |
---|---|---|
H47N | inactive mutant | Loxosceles reclusa |
H47N | inactive mutant | Loxosceles laeta |
H47N | inactive mutant | Loxosceles arizonica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Loxosceles reclusa | - |
- |
extracellular | - |
Loxosceles laeta | - |
- |
extracellular | - |
Loxosceles arizonica | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-lysophosphatidylcholine + H2O | Loxosceles reclusa | - |
2-lysophosphatidate + choline | - |
? | |
2-lysophosphatidylcholine + H2O | Loxosceles laeta | - |
2-lysophosphatidate + choline | - |
? | |
2-lysophosphatidylcholine + H2O | Loxosceles arizonica | - |
2-lysophosphatidate + choline | - |
? | |
additional information | Loxosceles reclusa | the spider venom enzyme cleaves the substrates sphingomyelin and lysophosphatidylcholine in mammalian tissues, releasing the choline head group | ? | - |
? | |
additional information | Loxosceles laeta | the spider venom enzyme cleaves the substrates sphingomyelin and lysophosphatidylcholine in mammalian tissues, releasing the choline head group | ? | - |
? | |
additional information | Loxosceles arizonica | the spider venom enzyme cleaves the substrates sphingomyelin and lysophosphatidylcholine in mammalian tissues, releasing the choline head group | ? | - |
? | |
sphingomyelin + H2O | Loxosceles reclusa | - |
ceramide phosphate + choline | - |
? | |
sphingomyelin + H2O | Loxosceles laeta | - |
ceramide phosphate + choline | - |
? | |
sphingomyelin + H2O | Loxosceles arizonica | - |
ceramide phosphate + choline | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Loxosceles arizonica | Q4ZFU2 | - |
- |
Loxosceles laeta | - |
- |
- |
Loxosceles reclusa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged recombinant enzyme from Escherichia coli strain BL21(lDE3) by nickel affinity chromatography | Loxosceles reclusa |
recombinant N-terminally His-tagged recombinant enzyme from Escherichia coli strain BL21(lDE3) by nickel affinity chromatography | Loxosceles laeta |
recombinant N-terminally His-tagged recombinant enzyme from Escherichia coli strain BL21(lDE3) by nickel affinity chromatography | Loxosceles arizonica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Loxosceles reclusa | - |
venom | - |
Loxosceles laeta | - |
venom | - |
Loxosceles arizonica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-octanoyl-2-hydroxy-sn-glycero-3-phosphocholine + H2O | Loxosceles whole venoms cleanly convert 8:0 LPC substrate to 8:0 CPA product | Loxosceles reclusa | 1-octanoyl-sn-glycero-2,3-cyclic-phosphate + choline | - |
? | |
1-octanoyl-2-hydroxy-sn-glycero-3-phosphocholine + H2O | Loxosceles whole venoms cleanly convert 8:0 LPC substrate to 8:0 CPA product | Loxosceles laeta | 1-octanoyl-sn-glycero-2,3-cyclic-phosphate + choline | - |
? | |
1-octanoyl-2-hydroxy-sn-glycero-3-phosphocholine + H2O | Loxosceles whole venoms cleanly convert 8:0 LPC substrate to 8:0 CPA product | Loxosceles arizonica | 1-octanoyl-sn-glycero-2,3-cyclic-phosphate + choline | - |
? | |
1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine + H2O | Loxosceles whole venoms and recombinant enzyme | Loxosceles reclusa | 1-palmitoyl-sn-glycero-2,3-cyclic-phosphate + choline | - |
? | |
1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine + H2O | Loxosceles whole venoms and recombinant enzyme | Loxosceles laeta | 1-palmitoyl-sn-glycero-2,3-cyclic-phosphate + choline | - |
? | |
1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine + H2O | Loxosceles whole venoms and recombinant enzyme | Loxosceles arizonica | 1-palmitoyl-sn-glycero-2,3-cyclic-phosphate + choline | - |
? | |
2-lysophosphatidylcholine + H2O | - |
Loxosceles reclusa | 2-lysophosphatidate + choline | - |
? | |
2-lysophosphatidylcholine + H2O | - |
Loxosceles laeta | 2-lysophosphatidate + choline | - |
? | |
2-lysophosphatidylcholine + H2O | - |
Loxosceles arizonica | 2-lysophosphatidate + choline | - |
? | |
additional information | the spider venom enzyme cleaves the substrates sphingomyelin and lysophosphatidylcholine in mammalian tissues, releasing the choline head group | Loxosceles reclusa | ? | - |
? | |
additional information | the spider venom enzyme cleaves the substrates sphingomyelin and lysophosphatidylcholine in mammalian tissues, releasing the choline head group | Loxosceles laeta | ? | - |
? | |
additional information | the spider venom enzyme cleaves the substrates sphingomyelin and lysophosphatidylcholine in mammalian tissues, releasing the choline head group | Loxosceles arizonica | ? | - |
? | |
additional information | recombinant enzymes, as well as whole venoms from diverse Loxosceles species, exclusively catalyze transphosphatidylation rather than hydrolysis, forming cyclic phosphate products from both major substrates, e.g. 1-palmitoyl-sn-glycero-2,3-cyclic-phosphate. Cyclic phosphates have vastly different biological properties from their monoester counterparts, and they may be relevant to the pathology of brown spider envenomation | Loxosceles reclusa | ? | - |
? | |
additional information | recombinant enzymes, as well as whole venoms from diverse Loxosceles species, exclusively catalyze transphosphatidylation rather than hydrolysis, forming cyclic phosphate products from both major substrates, e.g. 1-palmitoyl-sn-glycero-2,3-cyclic-phosphate. Cyclic phosphates have vastly different biological properties from their monoester counterparts, and they may be relevant to the pathology of brown spider envenomation | Loxosceles laeta | ? | - |
? | |
additional information | recombinant enzymes, as well as whole venoms from diverse Loxosceles species, exclusively catalyze transphosphatidylation rather than hydrolysis, forming cyclic phosphate products from both major substrates, e.g. 1-palmitoyl-sn-glycero-2,3-cyclic-phosphate. Cyclic phosphates have vastly different biological properties from their monoester counterparts, and they may be relevant to the pathology of brown spider envenomation | Loxosceles arizonica | ? | - |
? | |
sphingomyelin + H2O | - |
Loxosceles reclusa | ceramide phosphate + choline | - |
? | |
sphingomyelin + H2O | - |
Loxosceles laeta | ceramide phosphate + choline | - |
? | |
sphingomyelin + H2O | - |
Loxosceles arizonica | ceramide phosphate + choline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
A1LB1 | - |
Loxosceles arizonica |
alphaIB2bi | - |
Loxosceles arizonica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Loxosceles reclusa |
37 | - |
assay at | Loxosceles laeta |
37 | - |
assay at | Loxosceles arizonica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Loxosceles reclusa |
8 | - |
assay at | Loxosceles laeta |
8 | - |
assay at | Loxosceles arizonica |