Literature summary for 3.1.4.41 extracted from

Ullah, A.; Magalhaes, G.S.; Masood, R.; Mariutti, R.B.; Coronado, M.A.; Murakami, M.T.; Barbaro, K.C.; Arni, R.K.
Crystallization and preliminary X-ray diffraction analysis of a novel sphingomyelinase D from Loxosceles gaucho venom (2014), Acta Crystallogr. Sect. F, 70, 1418-1420.

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Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression in Escherichia coli strain BL21 Star(DE3)pLysS	Loxosceles gaucho

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, hanging drop vapour diffusion method, mixxing of 0.001 ml of 15 mg/ml protein in phosphate buffer, pH 7.0, with 0.001 ml reservoir solution containing 0.1 M Tris-HCl, pH 8.2, 22% PEG 10000, equilibration against 0.5 ml reservoir solution, 18°C, X-ray diffraction structure determination and anaysis at 2.6 A resolution	Loxosceles gaucho

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Loxosceles gaucho	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-lysophosphatidylcholine + H2O	Loxosceles gaucho	-	lysophosphatidic acid + choline	-	?
sphingomyelin + H2O	Loxosceles gaucho	-	ceramide 1-phosphate + choline	-	?

Organism

Organism	UniProt	Comment	Textmining
Loxosceles gaucho	K9USW8	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzme from Escherichia coli strain BL21 Star(DE3)pLysS by affinity chromatography	Loxosceles gaucho

Source Tissue

Source Tissue	Comment	Organism	Textmining
venom	-	Loxosceles gaucho	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-lysophosphatidylcholine + H2O	-	Loxosceles gaucho	lysophosphatidic acid + choline	-	?
sphingomyelin + H2O	-	Loxosceles gaucho	ceramide 1-phosphate + choline	-	?

Synonyms

Synonyms	Comment	Organism
LgRec1	-	Loxosceles gaucho
SMase D	-	Loxosceles gaucho
sphingomyelinase D	-	Loxosceles gaucho

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Release 2023.1 (January 2023)