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Literature summary for 3.1.4.41 extracted from

  • de Giuseppe, P.O.; Ullah, A.; Silva, D.T.; Gremski, L.H.; Wille, A.C.; Chaves Moreira, D.; Ribeiro, A.S.; Chaim, O.M.; Murakami, M.T.; Veiga, S.S.; Arni, R.K.
    Structure of a novel class II phospholipase D: catalytic cleft is modified by a disulphide bridge (2011), Biochem. Biophys. Res. Commun., 409, 622-627.
    View publication on PubMed
Search for more literature for 3.1.4.41

Cloned(Commentary)

Cloned (Comment) Organism
enzyme expression in Escherichia coli strain BL21(DE3)pLysS Loxosceles intermedia

Crystallization (Commentary)

Crystallization (Comment) Organism
purified PLD, sitting drop vapor diffusion method, 0.002 ml of 17 mg/ml protein solution is mixed with 0.002 ml of reservoir solution containing 0.1 M Tris-HCl, pH 7.5, 40% v/v PEG 200, and equilibration over 1 ml reservoir solution, 18°C, X-ray diffraction structure determination and analysis at 1.7 A resolution Loxosceles intermedia

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Loxosceles intermedia
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Loxosceles intermedia

Organism

Organism UniProt Comment Textmining
Loxosceles intermedia
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Loxosceles intermedia
-

Subunits

Subunits Comment Organism
More structure molecular modeling, overview Loxosceles intermedia

Synonyms

Synonyms Comment Organism
class II phospholipase D
-
 Loxosceles intermedia
dermonecrotic toxin isoform 1
-
 Loxosceles intermedia
LiRecDT1
-
 Loxosceles intermedia
PLD
-
 Loxosceles intermedia

General Information

General Information Comment Organism
additional information active site structure, the highly conserved residue Trp230 is important in substrate binding, overview Loxosceles intermedia