Cloned (Comment) | Organism |
---|---|
expression of His6-tagged wild-type and mutant H12A phospholipase Ds in Escherichia coli strain BL21(DE3) | Loxosceles intermedia |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and H12A mutant enzymes, 0.002 ml of protein solution containing 17 mg/ml wild type enzyme and 9 mg/ml H12A mutant, respectively, are mixed with 0.002 ml of reservoir solution containing 0.1 M Tris-HCl, pH 7.5, 40% v/v PEG 200 for the wild-type enzyme, and 0.1 M Tris-HCl, pH 7.5 and 35% v/v PEG 200 for the H12A mutant, equilibration over 1 ml reservoir solution, X-ray diffraction structure determination and analysis at 1.95 and 1.6 A resolution, respectively | Loxosceles intermedia |
Protein Variants | Comment | Organism |
---|---|---|
H12A | site-directed mutagenesis | Loxosceles intermedia |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Loxosceles intermedia | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Loxosceles intermedia | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and mutant H12A phospholipase Ds from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Loxosceles intermedia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Loxosceles intermedia | - |
Synonyms | Comment | Organism |
---|---|---|
class II phospholipase D | - |
Loxosceles intermedia |
General Information | Comment | Organism |
---|---|---|
physiological function | phospholipases D are the major dermonecrotic component of Loxosceles intermedia venom and catalyze the hydrolysis of phospholipids, resulting in the formation of lipid mediators such as ceramide-1-phosphate and lysophosphatidic acid which can induce pathological and biological responses | Loxosceles intermedia |