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Literature summary for 3.1.4.40 extracted from

  • Kean, E.L.; Bighouse, K.J.
    Cytidine 5-monophosphosialic acid hydrolase. Subcellular location and properties (1974), J. Biol. Chem., 249, 7813-7823.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 46% inhibition at 25 mM Rattus norvegicus
5,5'-dithiobis(2-nitrobenzoic acid) 16.3% inhibition at 25 mM Rattus norvegicus
cAMP 19% inhibition at 0.86 mM Rattus norvegicus
CDP 64% inhibition at 0.86 mM Rattus norvegicus
CMP 37% inhibition at 0.86 mM, 96% inhibition at 3.8 mM Rattus norvegicus
CTP 84% inhibition at 0.86 mM Rattus norvegicus
Cu2+ 38% reduction of enzyme activity at 10 mM Rattus norvegicus
cysteine 80% inhibition at 25 mM Rattus norvegicus
cytidine 6.9% inhibition at 0.86 mM Rattus norvegicus
diphosphate 41% inhibition at 0.86 mM, 82% inhibition at 6.5 Rattus norvegicus
dithiothreitol 84% inhibition by 25 mM reduced dithiothreitol, 0% inhibition by 25 mM oxidized dithiotreitol Rattus norvegicus
EDTA 50% inhibition at 0.2 mM Rattus norvegicus
GDPglucose competitive, 83% inhibition Rattus norvegicus
GDPmannose competitive, 85% inhibition Rattus norvegicus
glutathione 62% inhibition by 25 mM reduced glutathione, 15% inhibition by 25 mM oxidized glutathione Rattus norvegicus
N-ethylmaleimide 5.8% inhibition at 25 mM Rattus norvegicus
p-chloromercuribenzoate 7.2% inhibition at 25 mM Rattus norvegicus
TDPglucose competitive, 70% inhibition Rattus norvegicus
UDP 76% inhibition at 0.86 mM Rattus norvegicus
UDP-N-acetylglucosamine competitive, 58% inhibition Rattus norvegicus
UDPgalactose 31% inhibition at 0.86 mM Rattus norvegicus
UDPgalacturonic acid competitive, 79% inhibition Rattus norvegicus
UDPglucose competitive, 70% inhibition Rattus norvegicus
UDPglucuronic acid competitive, 76% inhibition Rattus norvegicus
UDPmannose competitive, 61% inhibition Rattus norvegicus
UMP 26% inhibition at 0.86 mM Rattus norvegicus
uridine 7.5% inhibition at 0.86 mM Rattus norvegicus
UTP 83% inhibition at 0.86 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
CMP-N-acylneuraminate
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Rattus norvegicus
-
-
nucleus
-
Rattus norvegicus 5634
-
plasma membrane activity in subfractions Rattus norvegicus 5886
-
plasma membrane of liver cells Rattus norvegicus 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ restores 20% of initial enzymatic activity when inhibited by EDTA Rattus norvegicus
Mg2+ restores 8% of initial enzymatic activity when inhibited by EDTA Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CMP-N-acylneuraminate + H2O Rattus norvegicus
-
?
-
ir

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
blood
-
Rattus norvegicus
-
bone
-
Rattus norvegicus
-
brain
-
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
intestinal mucosa
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
muscle skeletal Rattus norvegicus
-
pancreas
-
Rattus norvegicus
-
retina
-
Rattus norvegicus
-
spleen
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, plasma membranes, 1 year, microsomes, 3 months, no loss of activity Rattus norvegicus
0°C, for several days no loss of activity, by the 25th day 42% of initial activity remains Rattus norvegicus
repeated freezing and thawing, no influence of enzyme activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acylneuraminate + H2O
-
Rattus norvegicus CMP + N-acylneuraminate
-
ir
CMP-N-acylneuraminate + H2O
-
Rattus norvegicus ?
-
ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
in Tris-HCl buffer Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
EDTA
-
Rattus norvegicus