Cloned (Comment) | Organism |
---|---|
the sequence corresponding to the N-terminal 164 amino acids of the full length cDNA of phospholipase Dalpha from tomato fruit is cloned in pET28(b) vector and expressed in Escherichia coli as a His-tagged protein, transient expression of C2-YFP | Solanum lycopersicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | recombinant C2 domain shows micromolar affinity towards Ca2+ with a maximum of 2 high affinity binding sites, unique calcium binding pockets with high electro-negativity | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Solanum lycopersicum | interaction of PLDalpha C2 domain with synthetic unilamellar vesicles shows maximum affinity towards phosphatidic acid, and virtually no binding with phosphatidylcholine. Electrostatic, rather than a hydrophobic mode of interaction between C2 domain and the phospholipid vesicles. The binding towards phosphoinositides is reduced with increasing degree of phosphorylation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the C2 domain is phosphorylated. The binding towards phosphoinositides is reduced with increasing degree of phosphorylation | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | interaction of PLDalpha C2 domain with synthetic unilamellar vesicles shows maximum affinity towards phosphatidic acid, and virtually no binding with phosphatidylcholine. Electrostatic, rather than a hydrophobic mode of interaction between C2 domain and the phospholipid vesicles. The binding towards phosphoinositides is reduced with increasing degree of phosphorylation | Solanum lycopersicum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phospholipase Dalpha | - |
Solanum lycopersicum |
General Information | Comment | Organism |
---|---|---|
physiological function | phospholipase Dalpha is involved in fruit development | Solanum lycopersicum |