Cloned (Comment) | Organism |
---|---|
gene HaPLDalpha, cloning from leaf DNA, DNA and amino acid sequence determination and analysis, expression of His-tagged PLDalpha in Escherichia coli | Helianthus annuus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | recombinant PLDalpha, dependent on | Helianthus annuus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helianthus annuus | - |
gene HaPLDalpha | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged PLDalpha from Escherichia coli by nickel affinity chromatography to homogeneity | Helianthus annuus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | PLDalpha | Helianthus annuus | - |
leaf | high expression level of PLDalpha | Helianthus annuus | - |
root | PLDalpha | Helianthus annuus | - |
seed | PLDalpha | Helianthus annuus | - |
stem | high expression level of PLDalpha | Helianthus annuus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | - |
Helianthus annuus | choline + phosphatidate | - |
? | |
phosphatidylethanolamine + H2O | - |
Helianthus annuus | ethanolamine + phosphatidate | - |
? | |
phosphatidylglycerol + H2O | - |
Helianthus annuus | glycerol + phosphatidate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D type phospholipase | - |
Helianthus annuus |
phospholipase Dalpha | - |
Helianthus annuus |
PLD | - |
Helianthus annuus |
PLDalpha | - |
Helianthus annuus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
recombinant PLDalpha | Helianthus annuus |
General Information | Comment | Organism |
---|---|---|
physiological function | D type phospholipases are enzymes that hydrolyze the head group of phospholipids to produce phosphatidic acid | Helianthus annuus |