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Literature summary for 3.1.4.4 extracted from

  • Nakazawa, Y.; Uchino, M.; Sagane, Y.; Sato, H.; Takano, K.
    Isolation and characterization of actinomycetes strains that produce phospholipase D having high transphosphatidylation activity (2009), Microbiol. Res., 164, 43-48.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Actinobacteria PLD performs two different reactions: a hydrolytic reaction and a transphosphatidylation reaction, the latter with a primary alcohol, both pathway share a common intermediate, mechanism, overview ?
-
?
phosphatidylcholine + H2O Actinobacteria
-
choline + phosphatidic acid
-
?
phosphatidylethanolamine + H2O Actinobacteria
-
ethanolamine + phosphatidic acid
-
?
phosphatidylglycerol + H2O Actinobacteria
-
glycerol + phosphatidic acid
-
?
phosphatidylserine + H2O Actinobacteria
-
serine + phosphatidic acid
-
?

Organism

Organism UniProt Comment Textmining
Actinobacteria
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diverse strains from soil, isolated from 25 soil samples in Setagaya, Tokyo, Japan
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
hydrolysis rate of various phospholipids, and conversion rates and selectivity of the various phospholipid-producing-transphosphatidylation reactions of the culture supernatants from selected strains, overview Actinobacteria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PLD performs two different reactions: a hydrolytic reaction and a transphosphatidylation reaction, the latter with a primary alcohol, both pathway share a common intermediate, mechanism, overview Actinobacteria ?
-
?
phosphatidylcholine + H2O
-
Actinobacteria choline + phosphatidic acid
-
?
phosphatidylethanolamine + H2O
-
Actinobacteria ethanolamine + phosphatidic acid
-
?
phosphatidylglycerol + H2O
-
Actinobacteria glycerol + phosphatidic acid
-
?
phosphatidylserine + H2O
-
Actinobacteria serine + phosphatidic acid
-
?

Synonyms

Synonyms Comment Organism
PLD
-
Actinobacteria

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
transphosphatidylation reaction assay at 37°C Actinobacteria
28
-
in vivo hydrolytic assay at Actinobacteria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
transphosphatidylation reaction assay at pH 5.5 Actinobacteria