Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Phorbol esters | activate isozyme PLD1beta | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of isozyme PLD2alpha in HEK-293 cells, overexpression of GFP-tagged isozyme in vitro | Homo sapiens |
expression of isozymes PLD1beta and PLD2alpha in HEK-293 cells, overexpression of GFP-tagged isozymes in vitro | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D16A | caspase 3 cleavage motif mutant of isozyme PLD2 | Homo sapiens |
D16A | site-directed mutagenesis of isozyme PLD2D16A, the mutant shows altered cleavage by caspase 3 compared to the wild-type enzyme | Homo sapiens |
D28A | caspase 3 cleavage motif mutant of isozyme PLD2 | Homo sapiens |
D28A | site-directed mutagenesis of isozyme PLD2D28A, the mutant does not show altered cleavage by caspase 3 compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | isozyme PLD2alpha contains functional caspase 3 cleavage sites; isozymes PLD1beta and PLD2alpha contain functional caspase 3 cleavage sites and identify the critical aspartate residues within PLD1beta that affect its activation by phorbol esters and attenuate phosphatidylcholine hydrolysis during apoptosis | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Homo sapiens | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | PLD isozymes are cleaved by caspase 3, cleavage site determination, isozyme PLD2alpha contains two consensus motifs for caspase 3 cleavage, DXXD or D/E, D/E, X, D, located in the loop region at DDVD545S between the PLD domains, mutational analysis, overview | ? | - |
? | |
additional information | Homo sapiens | PLD isozymes are cleaved by caspase 3, cleavage site determination, isozymes PLD1beta and PLD2alpha contain each two consensus motifs for caspase 3 cleavage, DXXD or D/E, D/E, X, D, located in the loop region at DDVD545S and DFID631R between the PLD domains, respectively, mutational analysis, overview | ? | - |
? | |
phosphatidylcholine + H2O | Homo sapiens | - |
choline + phosphatidic acid | phosphatidic acid plays a regulatory role in important cellular processes such as secretion, cellular shape change, and movement | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O14939 | PLD2; isozyme PLD2alpha | - |
Homo sapiens | Q13393 | PLD1; isozymes PLD1beta and PLD2alpha | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PLD isozymes are cleaved by caspase 3, cleavage site determination, isozyme PLD2alpha contains two consensus motifs for caspase 3 cleavage, DXXD or D/E, D/E, X, D, located in the loop region at DDVD545S between the PLD domains, mutational analysis, overview | Homo sapiens | ? | - |
? | |
additional information | PLD isozymes are cleaved by caspase 3, cleavage site determination, isozymes PLD1beta and PLD2alpha contain each two consensus motifs for caspase 3 cleavage, DXXD or D/E, D/E, X, D, located in the loop region at DDVD545S and DFID631R between the PLD domains, respectively, mutational analysis, overview | Homo sapiens | ? | - |
? | |
additional information | PLD also shows transphosphatidylation activity with 1-butanol resulting in formation of phosphatidylbutanol | Homo sapiens | ? | - |
? | |
phosphatidylcholine + H2O | - |
Homo sapiens | choline + phosphatidic acid | - |
? | |
phosphatidylcholine + H2O | - |
Homo sapiens | choline + phosphatidic acid | phosphatidic acid plays a regulatory role in important cellular processes such as secretion, cellular shape change, and movement | ? |
Synonyms | Comment | Organism |
---|---|---|
phospholipase D1beta | - |
Homo sapiens |
phospholipase D2alpha | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | phosphatidic acid plays a regulatory role in important cellular processes such as secretion, cellular shape change, and movement. PLD-based signaling also plays a pro-mitogenic and pro-survival role in cells and therefore anti-apoptotic | Homo sapiens |