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Literature summary for 3.1.4.4 extracted from

  • Dippe, M.; Ulbrich-Hofmann, R.
    Substrate specificity in phospholipid transformations by plant phospholipase D isoenzymes (2009), Phytochemistry, 70, 361-365.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Papaver somniferum
expressed in Escherichia coli Brassica oleracea var. capitata

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Papaver somniferum
Ca2+
-
Brassica oleracea var. capitata

Organism

Organism UniProt Comment Textmining
Brassica oleracea var. capitata O82549
-
-
Brassica oleracea var. capitata P55939
-
-
Papaver somniferum Q8VWE9
-
-
Papaver somniferum Q8W1B2
-
-

Purification (Commentary)

Purification (Comment) Organism
purification by Ca2+-mediated hydrophobic interaction chromatography and an anion exchange chromatography Papaver somniferum
purification by Ca2+-mediated hydrophobic interaction chromatography and an anion exchange chromatography Brassica oleracea var. capitata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.21
-
initial rate of transphosphatidylation, acceptor alcohol: D-Ser Brassica oleracea var. capitata
1.88
-
initial rate of transphosphatidylation, acceptor alcohol: L-Ser Brassica oleracea var. capitata
6.3
-
initial rate of hydrolysis, acceptor alcohol: L-Ser Brassica oleracea var. capitata
7.82
-
initial rate of hydrolysis, acceptor alcohol: D-Ser Brassica oleracea var. capitata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + D-serine for L- and D-serine a stereoselectivity of PLD is observed Papaver somniferum phosphatidylserine + choline
-
?
phosphatidylcholine + D-serine for L- and D-serine a stereoselectivity of PLD is observed Brassica oleracea var. capitata phosphatidylserine + choline
-
?
phosphatidylcholine + ethanolamine preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol Papaver somniferum phosphatidylethanolamine + choline
-
?
phosphatidylcholine + ethanolamine preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-Ser Brassica oleracea var. capitata phosphatidylethanolamine + choline
-
?
phosphatidylcholine + ethanolamine preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine Papaver somniferum phosphatidylethanolamine + choline
-
?
phosphatidylcholine + ethanolamine preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine Brassica oleracea var. capitata phosphatidylethanolamine + choline
-
?
phosphatidylcholine + glycerol preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol Papaver somniferum phosphatidylglycerol + choline
-
?
phosphatidylcholine + glycerol preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-Ser Brassica oleracea var. capitata phosphatidylglycerol + choline
-
?
phosphatidylcholine + glycerol preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine Papaver somniferum phosphatidylglycerol + choline
-
?
phosphatidylcholine + glycerol preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine Brassica oleracea var. capitata phosphatidylglycerol + choline
-
?
phosphatidylcholine + H2O the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine Brassica oleracea var. capitata phosphatidate + choline
-
?
phosphatidylcholine + H2O the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol Papaver somniferum phosphatidate + choline
-
?
phosphatidylcholine + H2O the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol equal to phosphatidylcholine higher than phosphatidylinositol higher than phosphatidylserine Brassica oleracea var. capitata phosphatidate + choline
-
?
phosphatidylcholine + H2O the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol Papaver somniferum phosphatidate + choline
-
?
phosphatidylcholine + L-serine for L- and D-serine a stereoselectivity of PLD is observed, preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine Papaver somniferum phosphatidylserine + choline
-
?
phosphatidylcholine + L-serine for L- and D-serine a stereoselectivity of PLD is observed, preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine Brassica oleracea var. capitata phosphatidylserine + choline
-
?
phosphatidylcholine + L-serine preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-Ser Brassica oleracea var. capitata phosphatidylserine + choline
-
?
phosphatidylethanolamine + H2O the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine Brassica oleracea var. capitata phosphatidate + ethanolamine
-
?
phosphatidylethanolamine + H2O the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol Papaver somniferum phosphatidate + ethanolamine
-
?
phosphatidylethanolamine + H2O the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol higher than phosphatidylserine Brassica oleracea var. capitata phosphatidate + ethanolamine
-
?
phosphatidylethanolamine + H2O the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol Papaver somniferum phosphatidate + ethanolamine
-
?
phosphatidylglycerol + H2O the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine Brassica oleracea var. capitata phosphatidate + glycerol
-
?
phosphatidylglycerol + H2O the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol Papaver somniferum phosphatidate + glycerol
-
?
phosphatidylglycerol + H2O the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol equal to phosphatidylcholine higher than phosphatidylinositol higher than phosphatidylserine Brassica oleracea var. capitata phosphatidate + glycerol
-
?
phosphatidylglycerol + H2O the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol Papaver somniferum phosphatidate + glycerol
-
?
phosphatidylinositol + H2O the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine Brassica oleracea var. capitata phosphatidate + inositol
-
?
phosphatidylinositol + H2O the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol Papaver somniferum phosphatidate + inositol
-
?
phosphatidylinositol + H2O the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol higher than phosphatidylserine Brassica oleracea var. capitata phosphatidate + inositol
-
?
phosphatidylinositol + H2O the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol Papaver somniferum phosphatidate + inositol
-
?
phosphatidylserine + H2O the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine Brassica oleracea var. capitata phosphatidate + serine
-
?
phosphatidylserine + H2O the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol higher than phosphatidylserine Brassica oleracea var. capitata phosphatidate + serine
-
?
phosphatidylserine + H2O the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol Papaver somniferum phosphatidate + serine
-
?

Synonyms

Synonyms Comment Organism
cPLD1
-
Brassica oleracea var. capitata
cPLD2
-
Brassica oleracea var. capitata
phospholipase D
-
Papaver somniferum
phospholipase D
-
Brassica oleracea var. capitata
pPLD1
-
Papaver somniferum
pPLD2
-
Papaver somniferum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Papaver somniferum
30
-
assay at Brassica oleracea var. capitata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
assay at Papaver somniferum
5.5
-
assay at Brassica oleracea var. capitata