Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Papaver somniferum |
expressed in Escherichia coli | Brassica oleracea var. capitata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Papaver somniferum | |
Ca2+ | - |
Brassica oleracea var. capitata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica oleracea var. capitata | O82549 | - |
- |
Brassica oleracea var. capitata | P55939 | - |
- |
Papaver somniferum | Q8VWE9 | - |
- |
Papaver somniferum | Q8W1B2 | - |
- |
Purification (Comment) | Organism |
---|---|
purification by Ca2+-mediated hydrophobic interaction chromatography and an anion exchange chromatography | Papaver somniferum |
purification by Ca2+-mediated hydrophobic interaction chromatography and an anion exchange chromatography | Brassica oleracea var. capitata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.21 | - |
initial rate of transphosphatidylation, acceptor alcohol: D-Ser | Brassica oleracea var. capitata |
1.88 | - |
initial rate of transphosphatidylation, acceptor alcohol: L-Ser | Brassica oleracea var. capitata |
6.3 | - |
initial rate of hydrolysis, acceptor alcohol: L-Ser | Brassica oleracea var. capitata |
7.82 | - |
initial rate of hydrolysis, acceptor alcohol: D-Ser | Brassica oleracea var. capitata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + D-serine | for L- and D-serine a stereoselectivity of PLD is observed | Papaver somniferum | phosphatidylserine + choline | - |
? | |
phosphatidylcholine + D-serine | for L- and D-serine a stereoselectivity of PLD is observed | Brassica oleracea var. capitata | phosphatidylserine + choline | - |
? | |
phosphatidylcholine + ethanolamine | preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol | Papaver somniferum | phosphatidylethanolamine + choline | - |
? | |
phosphatidylcholine + ethanolamine | preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-Ser | Brassica oleracea var. capitata | phosphatidylethanolamine + choline | - |
? | |
phosphatidylcholine + ethanolamine | preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine | Papaver somniferum | phosphatidylethanolamine + choline | - |
? | |
phosphatidylcholine + ethanolamine | preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine | Brassica oleracea var. capitata | phosphatidylethanolamine + choline | - |
? | |
phosphatidylcholine + glycerol | preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol | Papaver somniferum | phosphatidylglycerol + choline | - |
? | |
phosphatidylcholine + glycerol | preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-Ser | Brassica oleracea var. capitata | phosphatidylglycerol + choline | - |
? | |
phosphatidylcholine + glycerol | preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine | Papaver somniferum | phosphatidylglycerol + choline | - |
? | |
phosphatidylcholine + glycerol | preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine | Brassica oleracea var. capitata | phosphatidylglycerol + choline | - |
? | |
phosphatidylcholine + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine | Brassica oleracea var. capitata | phosphatidate + choline | - |
? | |
phosphatidylcholine + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol | Papaver somniferum | phosphatidate + choline | - |
? | |
phosphatidylcholine + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol equal to phosphatidylcholine higher than phosphatidylinositol higher than phosphatidylserine | Brassica oleracea var. capitata | phosphatidate + choline | - |
? | |
phosphatidylcholine + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol | Papaver somniferum | phosphatidate + choline | - |
? | |
phosphatidylcholine + L-serine | for L- and D-serine a stereoselectivity of PLD is observed, preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine | Papaver somniferum | phosphatidylserine + choline | - |
? | |
phosphatidylcholine + L-serine | for L- and D-serine a stereoselectivity of PLD is observed, preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine | Brassica oleracea var. capitata | phosphatidylserine + choline | - |
? | |
phosphatidylcholine + L-serine | preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-Ser | Brassica oleracea var. capitata | phosphatidylserine + choline | - |
? | |
phosphatidylethanolamine + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine | Brassica oleracea var. capitata | phosphatidate + ethanolamine | - |
? | |
phosphatidylethanolamine + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol | Papaver somniferum | phosphatidate + ethanolamine | - |
? | |
phosphatidylethanolamine + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol higher than phosphatidylserine | Brassica oleracea var. capitata | phosphatidate + ethanolamine | - |
? | |
phosphatidylethanolamine + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol | Papaver somniferum | phosphatidate + ethanolamine | - |
? | |
phosphatidylglycerol + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine | Brassica oleracea var. capitata | phosphatidate + glycerol | - |
? | |
phosphatidylglycerol + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol | Papaver somniferum | phosphatidate + glycerol | - |
? | |
phosphatidylglycerol + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol equal to phosphatidylcholine higher than phosphatidylinositol higher than phosphatidylserine | Brassica oleracea var. capitata | phosphatidate + glycerol | - |
? | |
phosphatidylglycerol + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol | Papaver somniferum | phosphatidate + glycerol | - |
? | |
phosphatidylinositol + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine | Brassica oleracea var. capitata | phosphatidate + inositol | - |
? | |
phosphatidylinositol + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol | Papaver somniferum | phosphatidate + inositol | - |
? | |
phosphatidylinositol + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol higher than phosphatidylserine | Brassica oleracea var. capitata | phosphatidate + inositol | - |
? | |
phosphatidylinositol + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol | Papaver somniferum | phosphatidate + inositol | - |
? | |
phosphatidylserine + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine | Brassica oleracea var. capitata | phosphatidate + serine | - |
? | |
phosphatidylserine + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol higher than phosphatidylserine | Brassica oleracea var. capitata | phosphatidate + serine | - |
? | |
phosphatidylserine + H2O | the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol higher than phosphatidylcholine higher than phosphatidylserine higher than phosphatidylinositol | Papaver somniferum | phosphatidate + serine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cPLD1 | - |
Brassica oleracea var. capitata |
cPLD2 | - |
Brassica oleracea var. capitata |
phospholipase D | - |
Papaver somniferum |
phospholipase D | - |
Brassica oleracea var. capitata |
pPLD1 | - |
Papaver somniferum |
pPLD2 | - |
Papaver somniferum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Papaver somniferum |
30 | - |
assay at | Brassica oleracea var. capitata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Papaver somniferum |
5.5 | - |
assay at | Brassica oleracea var. capitata |