Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CHAPS | relative activity: 201 | Streptomyces olivochromogenes | |
deoxycholic acid | relative activity: 246% | Streptomyces olivochromogenes | |
polyethylene-4-laurylether | relative activity: 125% | Streptomyces olivochromogenes | |
SDS | relative activity: 189 | Streptomyces olivochromogenes | |
Triton X-100 | relative activity: 429% | Streptomyces olivochromogenes | |
Tween-20 | relative activity: 257% | Streptomyces olivochromogenes | |
Tween-80 | relative activity: 249% | Streptomyces olivochromogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
phosphatidylcholine | Vmax: 650 micromol/min/mg | Streptomyces olivochromogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | enzyme activity is Ca2+-dependent | Streptomyces olivochromogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
SDS-PAGE | Streptomyces olivochromogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces olivochromogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a one-step chromatography on Sepharose CL-6B colum | Streptomyces olivochromogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + glycerol | - |
Streptomyces olivochromogenes | phosphatidylglycerol + choline | - |
? | |
phosphatidylcholine + H2O | - |
Streptomyces olivochromogenes | 1,2-diacylglycerophosphate + choline | - |
? | |
phosphatidylcholine + L-serine | - |
Streptomyces olivochromogenes | phosphatidylserine + choline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Streptomyces olivochromogenes |
Synonyms | Comment | Organism |
---|---|---|
phospholipase D | - |
Streptomyces olivochromogenes |
PLD | - |
Streptomyces olivochromogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
transphosphatidylation activity highest at | Streptomyces olivochromogenes |
75 | - |
maximal hydrolytic activity | Streptomyces olivochromogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 75 | - |
Streptomyces olivochromogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
hydrolytic and transphosphatidylation activity highest at | Streptomyces olivochromogenes |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 13 | - |
Streptomyces olivochromogenes |