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Literature summary for 3.1.4.4 extracted from

  • Simkhada, J.R.; Lee, H.J.; Jang, S.Y.; Kim, J.H.; Lee, H.C.; Sohng, J.K.; Yoo, J.C.
    A novel low molecular weight phospholipase D from Streptomyces sp. CS684 (2009), Biores. Technol., 100, 1388-1393.
    View publication on PubMed
Search for more literature for 3.1.4.4

Activating Compound

Activating Compound Comment Organism Structure
CHAPS relative activity: 195% Streptomyces sp.
deoxycholic acid relative activity: 273% Streptomyces sp.
Triton X-100 relative activity: 292% Streptomyces sp.
Tween-20 relative activity: 345% Streptomyces sp.
Tween-80 relative activity: 292% Streptomyces sp.

Application

Application Comment Organism
biotechnology the feature of the enzyme, to be highly active in moderate pH and temperature, makes PLD684 easy to handle and thus substantiate its potential biocatalytic application in the industrial scale Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
polyoxylethylene-4-laurylether relative activity: 3% Streptomyces sp.
SDS relative activity: 41% Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.16
-
 phosphatidylcholine pH 6.0, 45°C Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate fractionation, gel filtration with sepharose CL-6B, anion-exchange chromatography with DEAE-sepharose CL-6B and a second gel filtration with sepharose CL-6B Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + glycerol catalyzes the transphosphatidylation of glycerol, but not that of L-serine, myo-inositol or ethanolamine Streptomyces sp. phosphatidylglycerol + choline
-
 ?
phosphatidylcholine + H2O
-
 Streptomyces sp. choline + phosphatidate
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
phospholipase D
-
 Streptomyces sp.
PLD684
-
 Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 enzyme ist stable at or below 45°C when assayed after 40 h and 2 h, respectively Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 8 over 95% activity detected Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 activity is highly stable at 1°C when measured after 40 h Streptomyces sp.