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Literature summary for 3.1.4.4 extracted from
- Lysophosphatidylserine-induced functional switch of human cytochrome P450 1A2 and 2E1 from monooxygenase to phospholipase D (2008), Biochem. Biophys. Res. Commun., 376, 584-589.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| lysophosphatidylserine | stimulates PLD activity in a concentration-dependent manner and approximately 5fold and 8fold increases in CYP1A2 and CYP2E1 activities, respectively, are shown in the presence of 2 mol% of the lysophosphatidylserine when compared to a 100% phosphatidylcholine matrix. LysoPS also accompanies conformational changes in both CYP1A2 and CYP2E1 when assayed by circular dichroism | Homo sapiens |  |
| lysophosphatidylserine | stimulates PLD activity in a concentration-dependent manner and approximately 5fold and 8fold increases in CYP1A2 and CYP2E1 activities, respectively, are shown in the presence of 2 mol% of the lysophosphatidylserine when compared to a 100% phosphatidylcholine matrix. LysoPS also accompanies conformational changes in both CYP1A2 and CYP2E1 when assayed by circular dichroism | Rattus norvegicus | |
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | functional roles of CYP1A2 and CYP2E1 can be switched by interaction with a specific anionic lysophospholipid in vivo | Homo sapiens |
| molecular biology | functional roles of CYP1A2 and CYP2E1 can be switched by interaction with a specific anionic lysophospholipid in vivo | Rattus norvegicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Homo sapiens |
| expressed in Escherichia coli | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | PLD activity is decreased in the presence of components required for the monooxygenase (MMO) activity (reducing system), including 100% phosphatidylcholine membranes, NADPH-cytochrome P450 reductase and NADPH. Lysophosphatidylserine recoveres the PLD activity in a concentration-dependent manner | Homo sapiens | |
| additional information | PLD activity is decreased in the presence of components required for the monooxygenase (MMO) activity (reducing system), including 100% phosphatidylcholine membranes, NADPH-cytochrome P450 reductase and NADPH. Lysophosphatidylserine recoveres the PLD activity in a concentration-dependent manner | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using using Ni2+-affinity and ion-exchange chromatography | Homo sapiens |
| using using Ni2+-affinity and ion-exchange chromatography | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | - |
Homo sapiens | choline + phosphatidate | - |
? | |
| phosphatidylcholine + H2O | - |
Rattus norvegicus | choline + phosphatidate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phospholipase D | - |
Homo sapiens |
| phospholipase D | - |
Rattus norvegicus |
| PLD | - |
Homo sapiens |
| PLD | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Rattus norvegicus |
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