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Literature summary for 3.1.4.4 extracted from
- Identification and partial characterization of a highly active and stable phospholipase D from Brassica juncea seeds (2007), Int. J. Biol. Macromol., 40, 232-236.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme is independent of phosphatidylinositol-4,5-bisphosphate | Brassica juncea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 1.5 mM, 30% loss of activity | Brassica juncea |  |
| additional information | enzyme completely loses activity upon dialysis | Brassica juncea |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | about 40% stimulation in presence of at least 2 mM | Brassica juncea | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica juncea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial. Enzyme completely loses activity upon dialysis | Brassica juncea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Brassica juncea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 168.5 | - |
pH 5.5, 37°C, crude extract of seed | Brassica juncea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | - |
Brassica juncea | 1,2-diacylglycerophosphate + choline | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Brassica juncea |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 70 | more than 70% of maximum activity | Brassica juncea |
| 30 | 60 | more than 94% of maximum activity | Brassica juncea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
30 min, almost 100% rersidual activity | Brassica juncea |
| 70 | - |
30 min, 55% residual activity | Brassica juncea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Brassica juncea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | more than 73% of maximum activity | Brassica juncea |
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