Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of chimeras between TH-2PLD and PLD from a distict Streptomyces sp. | Streptomyces septatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values of chimeric enzymes | Streptomyces septatus | |
0.075 | - |
phosphatidyl-p-nitrophenol | pH 5.5, hydrolysis | Streptomyces septatus | |
1.16 | - |
phosphatidyl-p-nitrophenol | pH 5.5, transphosphatidylation | Streptomyces septatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces septatus | - |
recombinant enzyme | - |
Streptomyces septatus TH-2 | - |
recombinant enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme expressed in Escherichia coli | Streptomyces septatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidyl-p-nitrophenol + H2O | the N-terminal HKD motif contains the catalytic nucleophile, which attacks the phosphatidyl group of the substrate | Streptomyces septatus | phosphatidate + p-nitrophenol | - |
? | |
phosphatidyl-p-nitrophenol + H2O | the N-terminal HKD motif contains the catalytic nucleophile, which attacks the phosphatidyl group of the substrate | Streptomyces septatus TH-2 | phosphatidate + p-nitrophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TH-2PLD | - |
Streptomyces septatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover-numbers of chimeric enzymes | Streptomyces septatus | |
68.8 | - |
phosphatidyl-p-nitrophenol | pH 5.5, hydrolysis | Streptomyces septatus | |
174.8 | - |
phosphatidyl-p-nitrophenol | pH 5.5, transphosphatidylation | Streptomyces septatus |