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Literature summary for 3.1.4.4 extracted from

  • Lerchner, A.; Mansfeld, J.; Schaeffner, I.; Schoeps, R.; Beer, H.K.; Ulbrich-Hofmann, R.
    Two highly homologous phospholipase D isoenzymes from Papaver somniferum L. with different transphosphatidylation potential (2005), Biochim. Biophys. Acta, 1737, 94-101.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Papaver somniferum

Protein Variants

Protein Variants Comment Organism
A349E/E352Q strong suppression of hydrolysis and an increase in the transphosphatidylation activity Papaver somniferum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Papaver somniferum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ recombinant activity strongly increases in presence of Ca2+ in millimolar range Papaver somniferum
additional information no ativation of recombinant PLD1 in presemce of Zn2+ (0-50 mM) or Mg2+ (0-100 mM) Papaver somniferum
additional information no ativation of recombinant PLD2 in presemce of Zn2+ (0-50 mM) or Mg2+ (0-100 mM) Papaver somniferum

Organism

Organism UniProt Comment Textmining
Papaver somniferum Q8VWE9 L. Vajovecky
-
Papaver somniferum Q8W1B2 L. Vajovecky
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Papaver somniferum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.456
-
-
Papaver somniferum
0.5
-
-
Papaver somniferum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidyl-p-nitrophenol + H2O
-
Papaver somniferum phosphatidic acid + p-nitrophenol
-
?
phosphatidylcholine + H2O
-
Papaver somniferum 1,2-diacylglycerophosphate + choline
-
?

Synonyms

Synonyms Comment Organism
phospholipase D1
-
Papaver somniferum
phospholipase D2
-
Papaver somniferum
PLD1
-
Papaver somniferum
PLD2
-
Papaver somniferum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
at Ca2+ concentration above Papaver somniferum