Literature summary for 3.1.4.4 extracted from
Lim, S.K.; Choi, J.W.; Chung, M.H.; Lee, E.T.; Khang, Y.H.; Kim, S.D.; Nam, D.H.
Production and characterization of extracellular phospholipase D from Streptomyces sp. YU100 (2002), J. Microbiol. Biotechnol., 12, 189-195.
No PubMed abstract available
Activating Compound
Activating Compound |
Comment |
Organism |
Structure |
Triton X-100 |
optimal at 1.5% |
Streptomyces sp. |
|
General Stability
General Stability |
Organism |
relatively stable at high temperature and neutral pH-values, but significantly unstable in the alkaline range |
Streptomyces sp. |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
additional information |
no inhibitory effect of metal ions |
Streptomyces sp. |
|
Localization
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
extracellular |
secretion during early stage of growth |
Streptomyces sp. |
- |
- |
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
57000 |
- |
x * 57000, SDS-PAGE |
Streptomyces sp. |
Organism
Organism |
UniProt |
Comment |
Textmining |
Streptomyces sp. |
- |
YU100, optimal culture conditions |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
- |
Streptomyces sp. |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
phosphatidylcholine + H2O |
hydrolytic and transphosphatylation activity |
Streptomyces sp. |
1,2-diacylglycerophosphate + choline |
- |
? |
|
Subunits
Subunits |
Comment |
Organism |
? |
x * 57000, SDS-PAGE |
Streptomyces sp. |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
additional information |
- |
up to 60°C, increase of activity with increase of temperature |
Streptomyces sp. |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
4 |
- |
transphosphatidylation reaction |
Streptomyces sp. |
7 |
- |
hydrolytic reaction |
Streptomyces sp. |