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Literature summary for 3.1.4.4 extracted from

  • Ogino, C.; Negi, Y.; Matsumiya, T.; Nakaoka, K.; Kondo, A.; Kuroda, S.; Tokuyama, S.; Kikkawa, U.; Yamane, T.; Fukuda, H.
    Purification, characterization, and sequence determination of phospholipase D secreted by Streptoverticillium cinnamoneum (1999), J. Biochem., 125, 263-269.
    View publication on PubMed
Search for more literature for 3.1.4.4

Cloned(Commentary)

Cloned (Comment) Organism
-
 Streptomyces cinnamoneus

Inhibitors

Inhibitors Comment Organism Structure
Al3+ activation of transphosphatidylation Streptomyces cinnamoneus
Mn2+ substrate specific Streptomyces cinnamoneus

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ activation of transphosphatidylation Streptomyces cinnamoneus
Ca2+ stimulates only transphosphatidylation Streptomyces cinnamoneus
Mn2+ substrate specific Streptomyces cinnamoneus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 gel filtration, SDS-PAGE Streptomyces cinnamoneus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipid + H2O Streptomyces cinnamoneus phosphoric ester hydrolysis phosphatidic acid + alcohol
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces cinnamoneus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
complete to homogeneity Streptomyces cinnamoneus

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Streptomyces cinnamoneus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
468
-
-
 Streptomyces cinnamoneus

Storage Stability

Storage Stability Organism
55°C, pH 7.4, 24 h, 100% loss of activity Streptomyces cinnamoneus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + H2O
-
 Streptomyces cinnamoneus 1,2-diacylglycerophosphate + choline
-
 ?
phosphatidylethanolamine + H2O
-
 Streptomyces cinnamoneus 1,2-diacylglycerophosphate + ethanolamine
-
 ?
phosphatidylserine + H2O
-
 Streptomyces cinnamoneus 1,2-diacylglycerophosphate + serine
-
 ?
phospholipid + H2O phosphoric ester hydrolysis Streptomyces cinnamoneus phosphatidic acid + alcohol
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Streptomyces cinnamoneus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Streptomyces cinnamoneus