Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.4 extracted from

  • Okamura, S.; Yamashita, S.
    Purification and characterization of phosphatidylcholine phospholipase D from pig lung (1994), J. Biol. Chem., 269, 31207-31213.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
arachidonic acid only in presence of Mg2+ Sus scrofa
linoleic acid only in presence of Mg2+ Sus scrofa
lysophosphatidic acid
-
Sus scrofa
oleic acid only in presence of Mg2+ Sus scrofa
phosphatidylinositol 4,5-bisphosphate
-
Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
lysophosphatidylcholine
-
Sus scrofa
N-ethylmaleimide
-
Sus scrofa
palmitic acid
-
Sus scrofa
phosphatidic acid
-
Sus scrofa
phosphatidylethanolamine
-
Sus scrofa
phosphatidylinositol
-
Sus scrofa
stearic acid
-
Sus scrofa
sucrose monolaurate
-
Sus scrofa
Triton X-100
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
phosphatidylcholine
-
Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Sus scrofa 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ micromolar levels for maximal activity Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
190000
-
gel filtration, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipid + alcohol Sus scrofa transphosphaditylation phospholipid + alcohol
-
?
phospholipid + H2O Sus scrofa phosphoric ester hydrolysis phosphatidic acid + alcohol
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
complete to homogeneity Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.67
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + ethanol
-
Sus scrofa phosphatidylethanol + choline
-
?
phosphatidylcholine + ethanolamine
-
Sus scrofa phosphatidylethanolamine + choline
-
?
phosphatidylcholine + H2O
-
Sus scrofa 1,2-diacylglycerophosphate + choline
-
?
phosphatidylinositol + H2O
-
Sus scrofa 1,2-diacylglycerophosphate + inositol
-
?
phospholipid + alcohol transphosphaditylation Sus scrofa phospholipid + alcohol
-
?
phospholipid + H2O phosphoric ester hydrolysis Sus scrofa phosphatidic acid + alcohol
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
-
Sus scrofa