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Literature summary for 3.1.4.4 extracted from

  • Ueki, J.; Morioka, S.; Komari, T.; Kumashiro, T.
    Purification and characterization of phospholipase D (PLD) from rice (Oryza sativa L.) and cloning of cDNA for PLD from rice and maize (Zea mays L.) (1995), Plant Cell Physiol., 36, 903-914.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Zea mays
-
Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
phosphatidylcholine
-
Oryza sativa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Zea mays
Ca2+
-
Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000 82000 gel filtration, SDS-PAGE Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipid + H2O Zea mays phosphoric ester hydrolysis phosphatidic acid + alcohol
-
?
phospholipid + H2O Oryza sativa phosphoric ester hydrolysis phosphatidic acid + alcohol
-
?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
rice
-
Zea mays
-
maize
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
seed coat
-
Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lysophosphatidylcholine + H2O
-
Oryza sativa monoacylglycerophosphate + choline
-
?
phosphatidylcholine + H2O
-
Oryza sativa 1,2-diacylglycerophosphate + choline
-
?
phospholipid + H2O phosphoric ester hydrolysis Zea mays phosphatidic acid + alcohol
-
?
phospholipid + H2O phosphoric ester hydrolysis Oryza sativa phosphatidic acid + alcohol
-
?
sphingomyelin + H2O
-
Oryza sativa N-acylsphingosylphosphate + choline
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 37 stable for 30 min Oryza sativa
4 37 50% loss of activity at 50°C Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Oryza sativa